17-5899-42

antibody from Invitrogen Antibodies

Targeting: FCER1A FCE1A

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

17-5899-42

Provider

Invitrogen Antibodies

Product name

FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), APC, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

AER-37 (CRA1)

Vial size

100 Tests

Concentration

5 µL/Test

Storage

4° C, store in dark, DO NOT FREEZE!

References

Submitted references

The mechanistic and functional profile of the therapeutic anti-IgE antibody ligelizumab differs from omalizumab.

Gasser P, Tarchevskaya SS, Guntern P, Brigger D, Ruppli R, Zbären N, Kleinboelting S, Heusser C, Jardetzky TS, Eggel A
Nature communications 2020 Jan 8;11(1):165

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Identification of TNFR2 and IL-33 as therapeutic targets in localized fibrosis.

Izadi D, Layton TB, Williams L, McCann F, Cabrita M, Espirito Santo AI, Xie W, Fritzsche M, Colin-York H, Feldmann M, Midwood KS, Nanchahal J
Science advances 2019 Dec;5(12):eaay0370

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Structural basis of omalizumab therapy and omalizumab-mediated IgE exchange.

Pennington LF, Tarchevskaya S, Brigger D, Sathiyamoorthy K, Graham MT, Nadeau KC, Eggel A, Jardetzky TS
Nature communications 2016 May 19;7:11610

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A pilot study of omalizumab in eosinophilic esophagitis.

Loizou D, Enav B, Komlodi-Pasztor E, Hider P, Kim-Chang J, Noonan L, Taber T, Kaushal S, Limgala R, Brown M, Gupta R, Balba N, Goker-Alpan O, Khojah A, Alpan O
PloS one 2015;10(3):e0113483

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Dendritic cell-bound IgE functions to restrain allergic inflammation at mucosal sites.

Platzer B, Baker K, Vera MP, Singer K, Panduro M, Lexmond WS, Turner D, Vargas SO, Kinet JP, Maurer D, Baron RM, Blumberg RS, Fiebiger E
Mucosal immunology 2015 May;8(3):516-32

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Human Mucosal Mast Cells Capture HIV-1 and Mediate Viral trans-Infection of CD4+ T Cells.

Jiang AP, Jiang JF, Wei JF, Guo MG, Qin Y, Guo QQ, Ma L, Liu BC, Wang X, Veazey RS, Ding YB, Wang JH
Journal of virology 2015 Dec 30;90(6):2928-37

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Human Blood-Circulating Basophils Capture HIV-1 and Mediate Viral trans-Infection of CD4+ T Cells.

Jiang AP, Jiang JF, Guo MG, Jin YM, Li YY, Wang JH
Journal of virology 2015 Aug;89(15):8050-62

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MHC class II expression in human basophils: induction and lack of functional significance.

Voskamp AL, Prickett SR, Mackay F, Rolland JM, O'Hehir RE
PloS one 2013;8(12):e81777

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The signal peptide of the IgE receptor alpha-chain prevents surface expression of an immunoreceptor tyrosine-based activation motif-free receptor pool.

Platzer B, Fiebiger E
The Journal of biological chemistry 2010 May 14;285(20):15314-15323

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Generation, isolation, and maintenance of human mast cells and mast cell lines derived from peripheral blood or cord blood.

Rådinger M, Jensen BM, Kuehn HS, Kirshenbaum A, Gilfillan AM
Current protocols in immunology 2010 Aug;Chapter 7:Unit 7.37

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IgE- and FcepsilonRI-mediated migration of human basophils.

Suzukawa M, Hirai K, Iikura M, Nagase H, Komiya A, Yoshimura-Uchiyama C, Yamada H, Ra C, Ohta K, Yamamoto K, Yamaguchi M
International immunology 2005 Sep;17(9):1249-55

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Regulation of the human Fc epsilon RI alpha-chain distal promoter.

Hasegawa M, Nishiyama C, Nishiyama M, Akizawa Y, Takahashi K, Ito T, Furukawa S, Ra C, Okumura K, Ogawa H
Journal of immunology (Baltimore, Md. : 1950) 2003 Apr 1;170(7):3732-8

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IgE enhances Fc epsilon receptor I expression and IgE-dependent release of histamine and lipid mediators from human umbilical cord blood-derived mast cells: synergistic effect of IL-4 and IgE on human mast cell Fc epsilon receptor I expression and mediator release.

Yamaguchi M, Sayama K, Yano K, Lantz CS, Noben-Trauth N, Ra C, Costa JJ, Galli SJ
Journal of immunology (Baltimore, Md. : 1950) 1999 May 1;162(9):5455-65

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Functional expression of the high affinity receptor for IgE (FcepsilonRI) in human platelets and its' intracellular expression in human megakaryocytes.

Hasegawa S, Pawankar R, Suzuki K, Nakahata T, Furukawa S, Okumura K, Ra C
Blood 1999 Apr 15;93(8):2543-51

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Soluble human high-affinity receptor for IgE abrogates the IgE-mediated allergic reaction.

Ra C, Kuromitsu S, Hirose T, Yasuda S, Furuichi K, Okumura K
International immunology 1993 Jan;5(1):47-54

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The alpha subunit of the human IgE receptor (FcERI) is sufficient for high affinity IgE binding.

Hakimi J, Seals C, Kondas JA, Pettine L, Danho W, Kochan J
The Journal of biological chemistry 1990 Dec 25;265(36):22079-81

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Staining of normal human peripheral blood cells with Anti-Human IgE FITC (Product # 11-6986-42) and Mouse IgG2b K Isotype Control APC (Product # 17-4732-81) (left) or Anti-Human Fc epsilon Receptor I alpha (FceR1) APC (right). Cells in the lymphocyte gate were used for analysis (note: basophils reside in FCS/SSC position of lymphocytes).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 1 Mast cells and M2 macrophages are the predominant source of TNF in DD. ( A ) Scatter plot of cytokines secreted by freshly isolated nodular cells in monolayer culture for 24 hours ( n = 20 DD patients for TGF-beta1 and 40 for all other cytokines; lines represent mean). ( B ) Heatmap of marker genes of four major immune cell types in single-cell RNA-seq data, displaying average row scaled expression in log(UMI + 1). ( C ) t -distributed stochastic neighbor embedding ( t- SNE) embedding of single-cell RNA-seq of immune cells in Dupuytren's nodules ( n = 12 DD patients, k = 1033 cells). DCs, dendritic cells. ( D ) Scatter plot of flow cytometry analysis showing percentage of cell types as a proportion of the total population in Dupuytren's nodules ( n = 20 DD patients, line represents mean +- SEM). ( E ) Representative images of immunohistochemistry for macrophages (CD68) and mast cells (tryptase) in Dupuytren's nodules. Scale bars, 10 mum. ( F ) Dot plot mapping genes encoding chemokines and chemokine receptors to immune cell clusters (0 to 4). Expression in scaled(log(UMI + 1)). Pct.exp., percentage of cells in cluster-expressing gene. ( G ) Scatter plot of chemokines secreted by freshly isolated nodular cells in monolayer culture for 24 hours ( n = 40 DD patients; line represents mean). ( H ) Violin plots showing expression of genes in scaled(log(UMI + 1)) in immune cell clusters. P -adjust denotes adjusted P value (Benjamini-Hochberg correction) for TNF following diff