Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF8055 - Provider product page
- Provider
- R&D Systems
- Product name
- Human LKB1/STK11 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human LKB1/STK11 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q15831
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human LKB1/STK11 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human LKB1/STK11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8055) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LKB1/STK11 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Western Blot Shows Human LKB1/STK11 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and LKB1/STK11 knockout HEK293T cell line (KO). PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human LKB1/STK11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8055) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LKB1/STK11 at approximately 54 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- LKB1/STK11 in Human Breast. LKB1/STK11 was detected in formalin fixed paraffin-embedded sections of human breast tissue using Sheep Anti-Human LKB1/STK11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8055) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- LKB1/STK11 in Human Breast Cancer Tissue. LKB1/STK11 was detected in formalin fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human LKB1/STK11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8055) at 1 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to tumor cell nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.