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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- 101-M50 - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- TIE-2
- Antibody type
- Monoclonal
- Antigen
- human soluble extracellular TIE-2
- Description
- antibody purified from hybridoma supernatant
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
AMDLILINSLPLVSDAETSLTCIASGWRPHEPITI
GRDFEALMNQHQDPLEVTQDVTREWAKKVVWKREK
ASKINGAYFCEGRVRGEAIRIRTMKMRQQASFLPA
TLTMTVDKGDNVNISFKKVLIKEEDAVIYKNGSFI
HSVPRHEVPDILEVHLPHAQPQDAGVYSARYIGGN
LFTSAFTRLIVRRCEAQKWGPECNHLCTACMNNGV
CHEDTGECICPPGFMGRTCEKACELHTFGRTCKER
CSGQEGCKSYVFCLPDPYGCSCATGWKGLQCNEAC
HPGFYGPDCKLRCSCNNGEMCDRFQGCLCSPGWQG
LQCEREGIQRMTPKIVDLPDHIEVNSGKFNPICKA
SGWPLPTNEEMTLVKPDGTVLHPKDFNHTDHFSVA
IFTIHRILPPDSGVWVCSVNTVAGMVEKPFNISVK
VLPKPLNAPNVIDTGHNFAVINISSEPYFGDGPIK
SKKLLYKPVNHYEAWQHIQVTNEIVTLNYLEPRTE
YELCVQLVRRGEGGEGHPGPVRRFTTASIGLPPPR
GLNLLPKSQTTLNLTWQPIFPSSEDDFYVEVERRS
VQKSDQQNIKVPGNLTSVLLNNLHPREQYVVRARV
NTKAQGEWSEDLTAWTLSDILPPQPENIKISNITH
SSAVISWTILDGYSISSITIRYKVQGKNEDQHVDV
KIKNATITQYQLKGLEPETAYQVDIFAENNIGSSN
PAFSHELVTRSDKTHTCPPCPAPELLGGPSVFLFP
PKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYV
DGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWL
NGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVY
TLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESN
GQPENNYKTTPPMLDSDGSFFLYSKLTVDKSRWQQ
GNVFSCSVMHEALHNHYTQKSLSLSPGK- Isotype
- IgG
- Antibody clone number
- (#tek2)
- Vial size
- 100 µl
- Storage
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
- Handling
- Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.
Submitted references Identification of proangiogenic TIE2-expressing monocytes (TEMs) in human peripheral blood and cancer.
Changes in serum soluble VEGFR-1 and Tie-2 receptors in colorectal cancer patients following surgical resections.
Identification of a soluble form of the angiopoietin receptor TIE-2 released from endothelial cells and present in human blood.
Soluble Tie2 and Flt1 extracellular domains in serum of patients with renal cancer and response to antiangiogenic therapy.
Venneri MA, De Palma M, Ponzoni M, Pucci F, Scielzo C, Zonari E, Mazzieri R, Doglioni C, Naldini L
Blood 2007 Jun 15;109(12):5276-85
Blood 2007 Jun 15;109(12):5276-85
Changes in serum soluble VEGFR-1 and Tie-2 receptors in colorectal cancer patients following surgical resections.
Chin KF, Greenman J, Reusch P, Gardiner E, Marme D, Monson J
Anticancer research 2004 Jul-Aug;24(4):2353-7
Anticancer research 2004 Jul-Aug;24(4):2353-7
Identification of a soluble form of the angiopoietin receptor TIE-2 released from endothelial cells and present in human blood.
Reusch P, Barleon B, Weindel K, Martiny-Baron G, Gödde A, Siemeister G, Marmé D
Angiogenesis 2001;4(2):123-31
Angiogenesis 2001;4(2):123-31
Soluble Tie2 and Flt1 extracellular domains in serum of patients with renal cancer and response to antiangiogenic therapy.
Harris AL, Reusch P, Barleon B, Hang C, Dobbs N, Marme D
Clinical cancer research : an official journal of the American Association for Cancer Research 2001 Jul;7(7):1992-7
Clinical cancer research : an official journal of the American Association for Cancer Research 2001 Jul;7(7):1992-7
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Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Effects of PMA treatment on TIE-2 . HUVECs (passage 1) were stimulated for the indicated periods of time with PMA at 25 ng/ml or left untreated. Western blot analysis for the presence of TIE-2 protein by immunoprecipitation using antibodies directed against the extracellular domain of human TIE-2 (IP: TEK16 [#101-M54]; Western: TEK2 [#101-M50]).
- Sample type
- HUVEC
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Quantification of soluble and cellular TIE-2 by sandwich ELISA. Stimulation of sTIE-2 release by PMA and basic FGF in comparison to untreated cells. For capturing anti-human TIE-2 Cl.16 [#101-M54] was used, for the detection a mixture of biotinylated anti-human TIE-2 Cl. 2 [#101-M50] and Cl.9 [#101-M52].
- Sample type
- HUVEC
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- IHC with cryo sections of human spleen.
- Sample type
- Human Spleen
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence staining (green) of cryo-sections of human placenta tissue (fixed shortly in 4% PFA) (A) with anti-human TIE2 #tek2 [0.5mg/ml, 1:100] (Cat# 101-M50) and (B) control without primary antibody [20x objective]. Signal is mainly found in endothelial cells of the placenta.
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- FACS analysis with human primary dermal microvascular endothelial cells (HDMEC).
- Sample type
- HDMEC
