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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AF2720 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Tie-2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and mouse Tie-2 when phosphorylated at Y992 in Western blots.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Transcriptome Profiling of Neovascularized Corneas Reveals miR-204 as a Multi-target Biotherapy Deliverable by rAAVs.
Pulmonary pericytes regulate lung morphogenesis.
Cooperation between ZEB2 and Sp1 promotes cancer cell survival and angiogenesis during metastasis through induction of survivin and VEGF.
Tie2 protects the vasculature against thrombus formation in systemic inflammation.
Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma.
Utilizing combinatorial engineering to develop Tie2 targeting antagonistic angiopoetin-2 ligands as candidates for anti-angiogenesis therapy.
Regulation of Angiopoietin Signalling by Soluble Tie2 Ectodomain and Engineered Ligand Trap.
The endothelial transcription factor ERG mediates Angiopoietin-1-dependent control of Notch signalling and vascular stability.
Insertion of scFv into the hinge domain of full-length IgG1 monoclonal antibody results in tetravalent bispecific molecule with robust properties.
Structural basis of Tie2 activation and Tie2/Tie1 heterodimerization.
Angiopoetin-2 Signals Do Not Mediate the Hypervascularization of Islets in Type 2 Diabetes.
Endothelial exocytosis of angiopoietin-2 resulting from CCM3 deficiency contributes to cerebral cavernous malformation.
Tie1 attenuation reduces murine atherosclerosis in a dose-dependent and shear stress-specific manner.
Ectopic expression of angiopoietin-1 promotes neuronal differentiation in neural progenitor cells through the Akt pathway.
Angiopoietins assemble distinct Tie2 signalling complexes in endothelial cell-cell and cell-matrix contacts.
Lu Y, Tai PWL, Ai J, Gessler DJ, Su Q, Yao X, Zheng Q, Zamore PD, Xu X, Gao G
Molecular therapy. Nucleic acids 2018 Mar 2;10:349-360
Molecular therapy. Nucleic acids 2018 Mar 2;10:349-360
Pulmonary pericytes regulate lung morphogenesis.
Kato K, Diéguez-Hurtado R, Park DY, Hong SP, Kato-Azuma S, Adams S, Stehling M, Trappmann B, Wrana JL, Koh GY, Adams RH
Nature communications 2018 Jun 22;9(1):2448
Nature communications 2018 Jun 22;9(1):2448
Cooperation between ZEB2 and Sp1 promotes cancer cell survival and angiogenesis during metastasis through induction of survivin and VEGF.
Ko D, Kim S
Oncotarget 2018 Jan 2;9(1):726-742
Oncotarget 2018 Jan 2;9(1):726-742
Tie2 protects the vasculature against thrombus formation in systemic inflammation.
Higgins SJ, De Ceunynck K, Kellum JA, Chen X, Gu X, Chaudhry SA, Schulman S, Libermann TA, Lu S, Shapiro NI, Christiani DC, Flaumenhaft R, Parikh SM
The Journal of clinical investigation 2018 Apr 2;128(4):1471-1484
The Journal of clinical investigation 2018 Apr 2;128(4):1471-1484
Impaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma.
Kim J, Park DY, Bae H, Park DY, Kim D, Lee CK, Song S, Chung TY, Lim DH, Kubota Y, Hong YK, He Y, Augustin HG, Oliver G, Koh GY
The Journal of clinical investigation 2017 Oct 2;127(10):3877-3896
The Journal of clinical investigation 2017 Oct 2;127(10):3877-3896
Utilizing combinatorial engineering to develop Tie2 targeting antagonistic angiopoetin-2 ligands as candidates for anti-angiogenesis therapy.
Shlamkovich T, Aharon L, Barton WA, Papo N
Oncotarget 2017 May 16;8(20):33571-33585
Oncotarget 2017 May 16;8(20):33571-33585
Regulation of Angiopoietin Signalling by Soluble Tie2 Ectodomain and Engineered Ligand Trap.
Alawo DOA, Tahir TA, Fischer M, Bates DG, Amirova SR, Brindle NPJ
Scientific reports 2017 Jun 16;7(1):3658
Scientific reports 2017 Jun 16;7(1):3658
The endothelial transcription factor ERG mediates Angiopoietin-1-dependent control of Notch signalling and vascular stability.
Shah AV, Birdsey GM, Peghaire C, Pitulescu ME, Dufton NP, Yang Y, Weinberg I, Osuna Almagro L, Payne L, Mason JC, Gerhardt H, Adams RH, Randi AM
Nature communications 2017 Jul 11;8:16002
Nature communications 2017 Jul 11;8:16002
Insertion of scFv into the hinge domain of full-length IgG1 monoclonal antibody results in tetravalent bispecific molecule with robust properties.
Bezabeh B, Fleming R, Fazenbaker C, Zhong H, Coffman K, Yu XQ, Leow CC, Gibson N, Wilson S, Stover CK, Wu H, Gao C, Dimasi N
mAbs 2017 Feb Mar;9(2):240-256
mAbs 2017 Feb Mar;9(2):240-256
Structural basis of Tie2 activation and Tie2/Tie1 heterodimerization.
Leppänen VM, Saharinen P, Alitalo K
Proceedings of the National Academy of Sciences of the United States of America 2017 Apr 25;114(17):4376-4381
Proceedings of the National Academy of Sciences of the United States of America 2017 Apr 25;114(17):4376-4381
Angiopoetin-2 Signals Do Not Mediate the Hypervascularization of Islets in Type 2 Diabetes.
Shah P, Lueschen N, Ardestani A, Oberholzer J, Olerud J, Carlsson PO, Maedler K
PloS one 2016;11(9):e0161834
PloS one 2016;11(9):e0161834
Endothelial exocytosis of angiopoietin-2 resulting from CCM3 deficiency contributes to cerebral cavernous malformation.
Jenny Zhou H, Qin L, Zhang H, Tang W, Ji W, He Y, Liang X, Wang Z, Yuan Q, Vortmeyer A, Toomre D, Fuh G, Yan M, Kluger MS, Wu D, Min W
Nature medicine 2016 Sep;22(9):1033-1042
Nature medicine 2016 Sep;22(9):1033-1042
Tie1 attenuation reduces murine atherosclerosis in a dose-dependent and shear stress-specific manner.
Woo KV, Qu X, Babaev VR, Linton MF, Guzman RJ, Fazio S, Baldwin HS
The Journal of clinical investigation 2011 Apr;121(4):1624-35
The Journal of clinical investigation 2011 Apr;121(4):1624-35
Ectopic expression of angiopoietin-1 promotes neuronal differentiation in neural progenitor cells through the Akt pathway.
Bai Y, Cui M, Meng Z, Shen L, He Q, Zhang X, Chen F, Xiao J
Biochemical and biophysical research communications 2009 Jan 9;378(2):296-301
Biochemical and biophysical research communications 2009 Jan 9;378(2):296-301
Angiopoietins assemble distinct Tie2 signalling complexes in endothelial cell-cell and cell-matrix contacts.
Saharinen P, Eklund L, Miettinen J, Wirkkala R, Anisimov A, Winderlich M, Nottebaum A, Vestweber D, Deutsch U, Koh GY, Olsen BR, Alitalo K
Nature cell biology 2008 May;10(5):527-37
Nature cell biology 2008 May;10(5):527-37
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse Phospho-Tie-2 (Y992) by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line transfected with mouse Tie-2 and untreated (-) or treated (+) with 600 ng/mL Recombinant Human Angiopoietin-1 (Catalog # 923-AN) for 5 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-Tie-2 (Y992) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2720) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Tie-2 (Y992) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Phospho-Tie-2 (Y992) in Human Kidney Tissue. Phospho-Tie-2 (Y992) in Human Kidney Tissue.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Tie-2 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were unstimulated (light orange filled histogram) or treated with 100 μM pervanadate for 15 minutes (dark orange filled histogram), then stained with Human/Mouse Phospho- Tie-2 (Y992) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2720) or control antibody (Catalog # AB-105-C, open histogram), followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
