Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- DM3509 - Provider product page
- Provider
- OriGene
- Product name
- TIE2 (TEK) mouse monoclonal antibody, clone Cl.2, Purified
- Antibody type
- Monoclonal
- Description
- TIE2 (TEK) mouse monoclonal antibody, clone Cl.2, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- TEK
- Isotype
- IgG
- Antibody clone number
- Cl.2
- Vial size
- 100 µg
- Concentration
- Lyophilized
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis for the presence of TiE-2 protein by immunoprecipitation using antibodies directed against the extracellular domain of Human TIE-2
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Quantification of soluble and cellular TIE-2 by Sandwich ELISA: ?A. CM and cell lysates from HUVECs treated with PMA (25ng/ml) or left untreated were analysed by Sandwich ELISA for the concentrations of sTIE-2 or TIE-2. For Capturing anti-Human TIE-2 Cl.16 ( DM3511) was used. for the detection a mixture of biotinylated anti-Human TIE-2 Cl.2 ( B) and Cl.9 ( DM3510).
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry with cryo-sections using?monoclonal anti-Human TIE-2 antibody
- Validation comment
- IHC