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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB3395 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3395, RRID:AB_1581285
- Product name
- TEK polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of TEK.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Tie2 receptor tyrosine kinase, a major mediator of tumor necrosis factor alpha-induced angiogenesis in rheumatoid arthritis.
Tie-2-dependent activation of RhoA and Rac1 participates in endothelial cell motility triggered by angiopoietin-1.
Expression of Tie-2 in human peripheral and autonomic nervous system.
DeBusk LM, Chen Y, Nishishita T, Chen J, Thomas JW, Lin PC
Arthritis and rheumatism 2003 Sep;48(9):2461-71
Arthritis and rheumatism 2003 Sep;48(9):2461-71
Tie-2-dependent activation of RhoA and Rac1 participates in endothelial cell motility triggered by angiopoietin-1.
Cascone I, Audero E, Giraudo E, Napione L, Maniero F, Philips MR, Collard JG, Serini G, Bussolino F
Blood 2003 Oct 1;102(7):2482-90
Blood 2003 Oct 1;102(7):2482-90
Expression of Tie-2 in human peripheral and autonomic nervous system.
Poncet S, Gasc JM, Janzer RC, Meyer S, Juillerat-Jeanneret L
Neuropathology and applied neurobiology 2003 Aug;29(4):361-9
Neuropathology and applied neurobiology 2003 Aug;29(4):361-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of TEK (arrow) using rabbit TEK polyclonal antibody (Cat # PAB3395). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the TEK gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with TEK polyclonal antibody (Cat # PAB3395) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
