Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 19157-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Tie2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: RRMAQAFQN VREEPAVQFN SGTLALNRKV KNNPDPTIYP VLDWNDIKFQ DVIGEGNFGQ VLKARIKKDG LRMDAAIKRM KEYASKDDHR DFAGELEVLC KLGHHPNIIN LLGACEHRGY LYLAIEYAPH GNLLDFLRKS RVLETDPAFA IANSTASTLS SHHLLHFAAD VARGMDYLSQ KQFIHRDLAA RNILVGENYV AKIADFGLSR GQEVYVKKTM GRLPVRWMAI ESLNYSVYTT NSDVWSYGVL LWEIVSLGGT PYCGMTCAEL YEKLPQGYRL EKPLNCDDEV YDLMRQCWRE KPYERPSFAQ ILVSLNRMLE ERKTYVNTTL YEKFTYAGID CSAEEAA (779-1124 aa encoded by BC035514)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.22 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse lung tissue were subjected to SDS PAGE followed by western blot with 19157-1-AP (Tie2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 19157-1-AP (Tie2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using (Product # 19157-1-AP) (Tie2 antibody) at dilution of 1:2,000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Tie2 (IP:19157-1-AP, 4ug; Detection:19157-1-AP 1:500) with mouse lung tissue lysate 4000ug.
