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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- PA5-28582 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TEK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HUVEC.
- Concentration
- 0.75 mg/mL
Submitted references Angiopoietin1 Deficiency in Hepatocytes Affects the Growth of Colorectal Cancer Liver Metastases (CRCLM).
Ibrahim NS, Lazaris A, Rada M, Petrillo SK, Huck L, Hussain S, Ouladan S, Gao ZH, Gregorieff A, Essalmani R, Seidah NG, Metrakos P
Cancers 2019 Dec 20;12(1)
Cancers 2019 Dec 20;12(1)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TIE2/TEK using 15 µg of HUVEC lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a TIE2/TEK polyclonal antibody (Product # PA5-28582) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using TEK Polyclonal Antibody (Product # PA5-28582). Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with TEK Polyclonal Antibody (Product # PA5-28582) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 IHC staining of ( A ) Ang2 and ( B ) Tie2 in RHGP and DHGP chemonaive lesions. ( C ) Positivity representing positive pixels staining quantified with Aperio software. RT- replacement tumor, DT- desmoplastic tumor. T- Tumor, L-liver, BV-Blood vessel, CT-Center of the tumor PT-Periphery of the tumor, DHGP ring-DR, dashed-lines represents the border between tumor region and livers, data are represented as the mean +/- SEM, and * significant p -value < 0.005, ** p -value < 0.0005 and *** p -value < 0.00005.
