DM3509

antibody from Acris Antibodies GmbH

Targeting: TEK CD202b, TIE-2, TIE2, VMCM, VMCM1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

DM3509

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#DM3509, RRID:AB_975403

Product name

anti CD202b / TEK

Antibody type

Monoclonal

Antigen

Recombinant Human soluble extracellular domain of TIE-2.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

Cl.2

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western Blot analysis for the presence of TiE-2 protein by immunoprecipitation using antibodies directed against the extracellular domain of Human TIE-2

ELISA

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Quantification of soluble and cellular TIE-2 by Sandwich ELISA: A. CM and cell lysates from HUVECs treated with PMA (25ng/ml) or left untreated were analysed by Sandwich ELISA for the concentrations of sTIE-2 or TIE-2. For Capturing anti-Human TIE-2 Cl.16 (Cat.-No DM3511) was used. for the detection a mixture of biotinylated anti-Human TIE-2 Cl.2 (Cat.-No DM3509B) and Cl.9 (Cat.-No DM3510).

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry with cryo-sections using monoclonal anti-Human TIE-2 antibody