Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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Validation data
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- Product number
- MA1-25358 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-ATF6 Monoclonal Antibody (70B1413)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA1-25358 detects ATF6 from human samples. MA1-25358 has been successfully used in Western blot procedures. The MA1-25358 immunogen is full length human protein.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 70B1413
- Vial size
- 50 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of ATF6 in NIH-3T3 cell lysate using an ATF6 monoclonal antibody (Product # MA1-25358) at a dilution of 3 µg/mL. A band corresponding to full-length ATF6 was detected. The ~36 kDa observed band may be an ATF6 breakdown/cleavage product.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of ATF6 was achieved by transfecting HeLa cells with ATF6 specific siRNAs (Silencer® select Product # s22688, s223543). Western blot analysis (Fig. a) was performed using whole cell extracts from the ATF6 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with ATF6 Monoclonal Antibody (Product # MA1-25358, 1:1000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to ATF6.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extract (30 µg lysate) of MCF7 (Lane 1), MDA-MB-231 (Lane 2), A549 (Lane 3), U87MG (Lane 4), SHSY5Y (Lane 5), NIH3T3 (Lane 6) and HeLa (Lane 7). The blot was probed with Anti-ATF6 Monoclonal Antibody (70B1413) (Product # MA1-25358, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 36 kDa band corresponding to the cleaved form of ATF6 was detected in all cell lines tested.