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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- MAB6762 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB6762, RRID:AB_10677248
- Product name
- ATF6 monoclonal antibody, clone 1-7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant ATF6.
- Isotype
- IgG
- Antibody clone number
- 1-7
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Identification and characterization of two novel spliced genes located in the orf47-orf46-orf45 gene locus of Kaposi's sarcoma-associated herpesvirus.
Transcriptional induction of mammalian ER quality control proteins is mediated by single or combined action of ATF6alpha and XBP1.
Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic reticulum stress.
Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers.
Chang PJ, Hung CH, Wang SS, Tsai PH, Shih YJ, Chen LY, Huang HY, Wei LH, Yen JB, Lin CL, Chen LW
Journal of virology 2014 Sep 1;88(17):10092-109
Journal of virology 2014 Sep 1;88(17):10092-109
Transcriptional induction of mammalian ER quality control proteins is mediated by single or combined action of ATF6alpha and XBP1.
Yamamoto K, Sato T, Matsui T, Sato M, Okada T, Yoshida H, Harada A, Mori K
Developmental cell 2007 Sep;13(3):365-76
Developmental cell 2007 Sep;13(3):365-76
Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic reticulum stress.
Haze K, Yoshida H, Yanagi H, Yura T, Mori K
Molecular biology of the cell 1999 Nov;10(11):3787-99
Molecular biology of the cell 1999 Nov;10(11):3787-99
Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers.
Hai TW, Liu F, Coukos WJ, Green MR
Genes & development 1989 Dec;3(12B):2083-90
Genes & development 1989 Dec;3(12B):2083-90
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of human cell extracts using ATF6 monoclonal antibody, clone 1-7 (Cat # MAB6762).Conversion of pATF6alpha (P) to pATF6alpha (N) in DTT- or tunicamycin-treated cells. Tm: 2 ug/mL tunicamycin (inhibitor of N-glycosylation). DTT : 1mM dithiothreitol (reducing reagent). The asterisk denotes an unglycosylated form of pATF6alpha (P).ATF6alpha is constitutively expressed as pATF6alpha (P) (~90-kDa protein), and converted to pATF6alpha (N) (>50-kDa protein) in ER-stressed cells.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- IP-Western blot analysis of human cell extracts using ATF6 monoclonal antibody, clone 1-7 (Cat # MAB6762).
- Validation comment
- Immunoprecipitation
