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antibody from Enzo Life Sciences
Targeting: ATF6
ATF6A
Western blot
Immunocytochemistry
Immunoprecipitation Immunohistochemistry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [23]
- Comments [0]
- Validations
- Immunocytochemistry [2]
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- Product number
- ALX-804-381-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-381-C100, RRID:AB_2050767
- Product name
- ATF6 monoclonal antibody (70B1413.1)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Protein G-affinity purified.
- Reactivity
- Human, Mouse, Rat, Hamster, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 70B1413.1
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20¡C.
Submitted references Resveratrol triggers ER stress-mediated apoptosis by disrupting N-linked glycosylation of proteins in ovarian cancer cells.
Response of myeloma to the proteasome inhibitor bortezomib is correlated with the unfolded protein response regulator XBP-1.
ATF6alpha induces XBP1-independent expansion of the endoplasmic reticulum.
Eif-2a protects brainstem motoneurons in a murine model of sleep apnea.
Cardiomyocyte apoptosis in autoimmune cardiomyopathy: mediated via endoplasmic reticulum stress and exaggerated by norepinephrine.
Acetaminophen induces ER dependent signaling in mouse liver.
Regulation of ERGIC-53 gene transcription in response to endoplasmic reticulum stress.
Nitric oxide-induced endoplasmic reticulum stress activates the expression of cargo receptor proteins and alters the glycoprotein transport to the Golgi complex.
Dynamic recruitment of transcription factors and epigenetic changes on the ER stress response gene promoters.
Endoplasmic reticulum stress and neurodegeneration in rats neonatally infected with borna disease virus.
Glia-specific activation of all pathways of the unfolded protein response in vanishing white matter disease.
Intrinsic capacities of molecular sensors of the unfolded protein response to sense alternate forms of endoplasmic reticulum stress.
Cytoprotective gene bi-1 is required for intrinsic protection from endoplasmic reticulum stress and ischemia-reperfusion injury.
Involvement of a novel Q-SNARE, D12, in quality control of the endomembrane system.
Flavivirus infection activates the XBP1 pathway of the unfolded protein response to cope with endoplasmic reticulum stress.
Intramembrane proteolytic cleavage by human signal peptide peptidase like 3 and malaria signal peptide peptidase.
3,3'-diindolylmethane (DIM) and its derivatives induce apoptosis in pancreatic cancer cells through endoplasmic reticulum stress-dependent upregulation of DR5.
Spinal cord endoplasmic reticulum stress associated with a microsomal accumulation of mutant superoxide dismutase-1 in an ALS model.
The unfolded protein response modulates toxicity of the expanded glutamine androgen receptor.
A signal peptide peptidase (SPP) reporter activity assay based on the cleavage of type II membrane protein substrates provides further evidence for an inverted orientation of the SPP active site relative to presenilin.
Underglycosylation of ATF6 as a novel sensing mechanism for activation of the unfolded protein response.
Kaposi's sarcoma-associated herpesvirus-infected primary effusion lymphoma has a plasma cell gene expression profile.
Requirement of the p38 mitogen-activated protein kinase signalling pathway for the induction of the 78 kDa glucose-regulated protein/immunoglobulin heavy-chain binding protein by azetidine stress: activating transcription factor 6 as a target for stress-induced phosphorylation.
Gwak H, Kim S, Dhanasekaran DN, Song YS
Cancer letters 2016 Feb 28;371(2):347-53
Cancer letters 2016 Feb 28;371(2):347-53
Response of myeloma to the proteasome inhibitor bortezomib is correlated with the unfolded protein response regulator XBP-1.
Ling SC, Lau EK, Al-Shabeeb A, Nikolic A, Catalano A, Iland H, Horvath N, Ho PJ, Harrison S, Fleming S, Joshua DE, Allen JD
Haematologica 2012 Jan;97(1):64-72
Haematologica 2012 Jan;97(1):64-72
ATF6alpha induces XBP1-independent expansion of the endoplasmic reticulum.
Bommiasamy H, Back SH, Fagone P, Lee K, Meshinchi S, Vink E, Sriburi R, Frank M, Jackowski S, Kaufman RJ, Brewer JW
Journal of cell science 2009 May 15;122(Pt 10):1626-36
Journal of cell science 2009 May 15;122(Pt 10):1626-36
Eif-2a protects brainstem motoneurons in a murine model of sleep apnea.
Zhu Y, Fenik P, Zhan G, Sanfillipo-Cohn B, Naidoo N, Veasey SC
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Feb 27;28(9):2168-78
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Feb 27;28(9):2168-78
Cardiomyocyte apoptosis in autoimmune cardiomyopathy: mediated via endoplasmic reticulum stress and exaggerated by norepinephrine.
Mao W, Fukuoka S, Iwai C, Liu J, Sharma VK, Sheu SS, Fu M, Liang CS
American journal of physiology. Heart and circulatory physiology 2007 Sep;293(3):H1636-45
American journal of physiology. Heart and circulatory physiology 2007 Sep;293(3):H1636-45
Acetaminophen induces ER dependent signaling in mouse liver.
Nagy G, Kardon T, Wunderlich L, Szarka A, Kiss A, Schaff Z, Bánhegyi G, Mandl J
Archives of biochemistry and biophysics 2007 Mar 15;459(2):273-9
Archives of biochemistry and biophysics 2007 Mar 15;459(2):273-9
Regulation of ERGIC-53 gene transcription in response to endoplasmic reticulum stress.
Renna M, Caporaso MG, Bonatti S, Kaufman RJ, Remondelli P
The Journal of biological chemistry 2007 Aug 3;282(31):22499-512
The Journal of biological chemistry 2007 Aug 3;282(31):22499-512
Nitric oxide-induced endoplasmic reticulum stress activates the expression of cargo receptor proteins and alters the glycoprotein transport to the Golgi complex.
Renna M, Faraonio R, Bonatti S, De Stefano D, Carnuccio R, Tajana G, Remondelli P
The international journal of biochemistry & cell biology 2006;38(12):2040-8
The international journal of biochemistry & cell biology 2006;38(12):2040-8
Dynamic recruitment of transcription factors and epigenetic changes on the ER stress response gene promoters.
Donati G, Imbriano C, Mantovani R
Nucleic acids research 2006;34(10):3116-27
Nucleic acids research 2006;34(10):3116-27
Endoplasmic reticulum stress and neurodegeneration in rats neonatally infected with borna disease virus.
Williams BL, Lipkin WI
Journal of virology 2006 Sep;80(17):8613-26
Journal of virology 2006 Sep;80(17):8613-26
Glia-specific activation of all pathways of the unfolded protein response in vanishing white matter disease.
van Kollenburg B, van Dijk J, Garbern J, Thomas AA, Scheper GC, Powers JM, van der Knaap MS
Journal of neuropathology and experimental neurology 2006 Jul;65(7):707-15
Journal of neuropathology and experimental neurology 2006 Jul;65(7):707-15
Intrinsic capacities of molecular sensors of the unfolded protein response to sense alternate forms of endoplasmic reticulum stress.
DuRose JB, Tam AB, Niwa M
Molecular biology of the cell 2006 Jul;17(7):3095-107
Molecular biology of the cell 2006 Jul;17(7):3095-107
Cytoprotective gene bi-1 is required for intrinsic protection from endoplasmic reticulum stress and ischemia-reperfusion injury.
Bailly-Maitre B, Fondevila C, Kaldas F, Droin N, Luciano F, Ricci JE, Croxton R, Krajewska M, Zapata JM, Kupiec-Weglinski JW, Farmer D, Reed JC
Proceedings of the National Academy of Sciences of the United States of America 2006 Feb 21;103(8):2809-14
Proceedings of the National Academy of Sciences of the United States of America 2006 Feb 21;103(8):2809-14
Involvement of a novel Q-SNARE, D12, in quality control of the endomembrane system.
Okumura AJ, Hatsuzawa K, Tamura T, Nagaya H, Saeki K, Okumura F, Nagao K, Nishikawa M, Yoshimura A, Wada I
The Journal of biological chemistry 2006 Feb 17;281(7):4495-506
The Journal of biological chemistry 2006 Feb 17;281(7):4495-506
Flavivirus infection activates the XBP1 pathway of the unfolded protein response to cope with endoplasmic reticulum stress.
Yu CY, Hsu YW, Liao CL, Lin YL
Journal of virology 2006 Dec;80(23):11868-80
Journal of virology 2006 Dec;80(23):11868-80
Intramembrane proteolytic cleavage by human signal peptide peptidase like 3 and malaria signal peptide peptidase.
Nyborg AC, Ladd TB, Jansen K, Kukar T, Golde TE
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2006 Aug;20(10):1671-9
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2006 Aug;20(10):1671-9
3,3'-diindolylmethane (DIM) and its derivatives induce apoptosis in pancreatic cancer cells through endoplasmic reticulum stress-dependent upregulation of DR5.
Abdelrahim M, Newman K, Vanderlaag K, Samudio I, Safe S
Carcinogenesis 2006 Apr;27(4):717-28
Carcinogenesis 2006 Apr;27(4):717-28
Spinal cord endoplasmic reticulum stress associated with a microsomal accumulation of mutant superoxide dismutase-1 in an ALS model.
Kikuchi H, Almer G, Yamashita S, Guégan C, Nagai M, Xu Z, Sosunov AA, McKhann GM 2nd, Przedborski S
Proceedings of the National Academy of Sciences of the United States of America 2006 Apr 11;103(15):6025-30
Proceedings of the National Academy of Sciences of the United States of America 2006 Apr 11;103(15):6025-30
The unfolded protein response modulates toxicity of the expanded glutamine androgen receptor.
Thomas M, Yu Z, Dadgar N, Varambally S, Yu J, Chinnaiyan AM, Lieberman AP
The Journal of biological chemistry 2005 Jun 3;280(22):21264-71
The Journal of biological chemistry 2005 Jun 3;280(22):21264-71
A signal peptide peptidase (SPP) reporter activity assay based on the cleavage of type II membrane protein substrates provides further evidence for an inverted orientation of the SPP active site relative to presenilin.
Nyborg AC, Jansen K, Ladd TB, Fauq A, Golde TE
The Journal of biological chemistry 2004 Oct 8;279(41):43148-56
The Journal of biological chemistry 2004 Oct 8;279(41):43148-56
Underglycosylation of ATF6 as a novel sensing mechanism for activation of the unfolded protein response.
Hong M, Luo S, Baumeister P, Huang JM, Gogia RK, Li M, Lee AS
The Journal of biological chemistry 2004 Mar 19;279(12):11354-63
The Journal of biological chemistry 2004 Mar 19;279(12):11354-63
Kaposi's sarcoma-associated herpesvirus-infected primary effusion lymphoma has a plasma cell gene expression profile.
Jenner RG, Maillard K, Cattini N, Weiss RA, Boshoff C, Wooster R, Kellam P
Proceedings of the National Academy of Sciences of the United States of America 2003 Sep 2;100(18):10399-404
Proceedings of the National Academy of Sciences of the United States of America 2003 Sep 2;100(18):10399-404
Requirement of the p38 mitogen-activated protein kinase signalling pathway for the induction of the 78 kDa glucose-regulated protein/immunoglobulin heavy-chain binding protein by azetidine stress: activating transcription factor 6 as a target for stress-induced phosphorylation.
Luo S, Lee AS
The Biochemical journal 2002 Sep 15;366(Pt 3):787-95
The Biochemical journal 2002 Sep 15;366(Pt 3):787-95
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis of ATF6 on 15µg of (A) Jurkat and (B)ÊNIH3T3 cell lysates using MAb to ATF6 (70B1413.1) (Prod. No. ALX-804-381). A band of ~90kDa was detected.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis of ATF6 using MAb to ATF6 (70B1413.1) (Prod. No. ALX-804-381). Method: Western blots were probed with 4µg/ml of MAb to ATF6 (70B1413.1), followed by an HRP-conjugated second step and visualized with PicoTect Western Blot Chemiluminescence Substrate. Film was exposed for 1 min. The top arrow corresponds to the ~90kDa form of ATF6 described as full-length in the literature. Lane 1: 293 cells transfected with full-length ATF6. Lane 2: 293 cells transfected with partial length ATF6 (aa 1-373). Lane 3: Untransfected 293 cells. The human full-length and partial length ATF6 plasmids are described in Luo and Lee (2002) [1].
