Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-22332 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RGS4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Raji, mouse cerebellum, rat brain.
- Concentration
- 0.43 mg/mL
Submitted references Regulator of G-Protein Signaling 4 (RGS4) Controls Morphine Reward by Glutamate Receptor Activation in the Nucleus Accumbens of Mouse Brain.
Kim J, Lee S, Kang S, Jeon TI, Kang MJ, Lee TH, Kim YS, Kim KS, Im HI, Moon C
Molecules and cells 2018 May 31;41(5):454-464
Molecules and cells 2018 May 31;41(5):454-464
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RGS4 using 50 µg mouse cerebellum lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a RGS4 polyclonal antibody (Product # PA5-22332) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RGS4 using 30 µg of Raji lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a RGS4 polyclonal antibody (Product # PA5-22332) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RGS4 Polyclonal Antibody (Product # PA5-22332). Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with RGS4 Polyclonal Antibody (Product # PA5-22332) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RGS4 Polyclonal Antibody detects RGS4 protein by western blot analysis. A. 50 µg rat brain lysate/extract.12% SDS-PAGE. RGS4 Polyclonal Antibody (Product # PA5-22332) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using RGS4 (Product # PA5-22332) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 1 RGS4 controlled morphine-induced reward in the NAc (A) Schematic representation of the shRNA-targeting RGS4 construct. (B) Injection target site in the mouse brain (NAc; red circle). (C) Representative photographs of eGFP expression in the NAc of mice injected with Lv-eGFP and Lv-shRNA-eGFP. (D) Representative immunoblots of RGS4 expression in the NAc of mice injected with Lv-eGFP and Lv-shRNA-eGFP. All data are expressed as means +- SEM; n = 6 for each group. (E) Schematic diagram of the schedule for the morphine CPP test. (F) Mice infected with Lv-shRNA-eGFP in the NAc showed an increased response to morphine (15 mg/kg, i.p.) in the CPP paradigm. All data are expressed as means +- SEM (n = 6 for each group). Data were analyzed with unpaired t -tests. * p < 0.05 vs. the Lv-eGFP infected group.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 Morphine induced the activation of ionotropic glutamate receptors in primary NAc/striatal neurons To examine the effects of morphine on glutamate receptors in NAc/striatal neurons, the neurons (9 DIV) were incubated with morphine (10 muM) for 3 days. (A) Representative photographs of immunoblots for RGS4, phospho-GluR1 (Ser831), total-GluR1, phospho-NR2A (Tyr1325), total-NR2A, phospho-CaMKII (Thr286), total-CaMKII, phospho-PKA-C (Thr197), and total-PKA-C. (B-F) Bar graphs showing semi-quantitative analyses of the phosphorylation levels of GluR1, NR2A, CaMKII, and PKA-C in NAc/striatal neurons after vehicle and morphine treatment. All data are expressed as means +- SEM; n = 6 for each group and were analyzed with unpaired t-tests. p < 0.05 vs . vehicle-treated control and ** p < 0.01 vs. vehicle-treated control.
