15681-1-AP

antibody from Invitrogen Antibodies

Targeting: KHK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15681-1-AP

Provider

Invitrogen Antibodies

Product name

KHK Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MEEKQILCV GLVVLDVISL VDKYPKEDSE IRCLSQRWQR GGNASNSCTI LSLLGAPCAF MGSMAPGHVA DFVLDDLRRY SVDLRYTVFQ TTGSVPIATV IINEASGSRT ILYYDRSLPD VSATDFEKVD LTQFKWIHIE GRNASEQVKM LQRIDAHNTR QPPEQKIRVS VEVEKPREEL FQLFGYGDVV FVSKDVAKHL GFQSAEEALR GLYGRVRKGA VLVCAWAEEG ADALGPDGKL LHSDAFPPPR VVDTLGAGDT FNASVIFSLS QGRSVQEALR FGCQVAGKKC GLQGFDGIV (1-298 aa encoded by BC006233 )

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.23 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 15681-1-AP (KHK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human liver tissue were subjected to SDS PAGE followed by western blot with 15681-1-AP (KHK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human liver tissue were subjected to SDS PAGE followed by western blot with 15681-1-AP (KHK antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 15681-1-AP (KHK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 15681-1-AP (KHK Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 15681-1-AP ( KHK Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 15681-1-AP ( KHK Antibody) at dilution of 1:400 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 15681-1-AP ( KHK Antibody) at dilution of 1:400 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-KHK (IP:15681-1-AP, 4ug; Detection:15681-1-AP 1:500) with mouse liver tissue lysate 4000ug.