AP7807a

antibody from Abgent

Targeting: ACVRL1 ACVRLK1, ALK1, HHT, HHT2, ORW2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP7807a

Provider

Abgent

Proper citation

Abgent Cat#AP7807a, RRID:AB_2242262

Product name

ACVRL1 Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

Submitted references

Regulation of endothelial barrier function by TGF-β type I receptor ALK5: potential role of contractile mechanisms and heat shock protein 90.

Antonov AS, Antonova GN, Fujii M, ten Dijke P, Handa V, Catravas JD, Verin AD
Journal of cellular physiology 2012 Feb;227(2):759-71

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ALK1 opposes ALK5/Smad3 signaling and expression of extracellular matrix components in human chondrocytes.

Finnson KW, Parker WL, ten Dijke P, Thorikay M, Philip A
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2008 Jun;23(6):896-906

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LC-MS/MS analysis of apical and basolateral plasma membranes of rat renal collecting duct cells.

Yu MJ, Pisitkun T, Wang G, Shen RF, Knepper MA
Molecular & cellular proteomics : MCP 2006 Nov;5(11):2131-45

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot of ACVRL1 Pab (Cat. #AP7807a). TOP LEFT: Mouse heart tissue lysate.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of ACVRL1 (arrow) using rabbit polyclonal ACVRL1 Antibody (N-term) (Cat. #AP7807a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACVRL1 gene (Lane 2) (Origene Technologies).

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"human chondrocytes (C28/I2 cells), transfected with empty vector (lane 1, 3) or ACVRL1(lane 2, 4). RIPA lysis buffer, 20 ug/lane of protein, primary antibody dilution 1:1000, blocking solution is 5% milk in TBST (lane 1 and 2), 5% BSA in TBST (lane 3 and 4). Data courtesy of Kenneth Finnson, Montreal General Hospital."

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."

Primary Ab dilution

1:50~100

Flow cytometry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Flow cytometric analysis of HepG2 cells using ACVRL1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50