Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-14921 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ACVRL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of mouse heart tissue lysate using an ACVRL1 polyclonal antibody (Product # PA5-14921).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ACVRL1 (arrow) using an ACVRL1 polyclonal antibody (Product # PA5-14921) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the ACVRL1 gene (Lane 2).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human chondrocytes (C28/I2 cells) transfected with empty vector (lane 1, 3) or ACVRL1 (lane 2, 4) using 20 µg/lane of protein. Cells were lysed with a RIPA lysis buffer, blocked with 5% milk in TBST (lane 1 and 2) and 5% BSA in TBST (lane 3 and 4), and probed with an ACVRL1 polyclonal antibody (Product # PA5-14921) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human chondrocytes (C28/I2 cells) transfected with empty vector (lane 1, 3) or ACVRL1 (lane 2, 4) using 20 µg/lane of protein. Cells were lysed with a RIPA lysis buffer, blocked with 5% milk in TBST (lane 1 and 2) and 5% BSA in TBST (lane 3 and 4), and probed with an ACVRL1 polyclonal antibody (Product # PA5-14921) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using an ACVRL1 polyclonal antibody (Product # PA5-14921), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HepG2 cells using an ACVRL1 polyclonal antibody (Product # PA5-14921) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody