AF370

antibody from R&D Systems

Targeting: ACVRL1 ACVRLK1, ALK1, HHT, HHT2, ORW2

Western blot (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

AF370

Provider

R&D Systems

Product name

Human ALK-1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human ALK-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant mouse ALK-1 is observed and less than 1% cross-reactivity with recombinant human (rh) Activin RIA and rhActivin RIB is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P37023


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Key role of the endothelial TGF-β/ALK1/endoglin signaling pathway in humans and rodents pulmonary hypertension.

Gore B, Izikki M, Mercier O, Dewachter L, Fadel E, Humbert M, Dartevelle P, Simonneau G, Naeije R, Lebrin F, Eddahibi S
PloS one 2014;9(6):e100310

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Anti-human activin receptor-like kinase 1 (ALK1) antibody attenuates bone morphogenetic protein 9 (BMP9)-induced ALK1 signaling and interferes with endothelial cell sprouting.

van Meeteren LA, Thorikay M, Bergqvist S, Pardali E, Stampino CG, Hu-Lowe D, Goumans MJ, ten Dijke P
The Journal of biological chemistry 2012 May 25;287(22):18551-61

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Functional analysis of the BMP9 response of ALK1 mutants from HHT2 patients: a diagnostic tool for novel ACVRL1 mutations.

Ricard N, Bidart M, Mallet C, Lesca G, Giraud S, Prudent R, Feige JJ, Bailly S
Blood 2010 Sep 2;116(9):1604-12

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Endoglin and activin receptor-like-kinase 1 are co-expressed in the distal vessels of the lung: implications for two familial vascular dysplasias, HHT and PAH.

Mahmoud M, Borthwick GM, Hislop AA, Arthur HM
Laboratory investigation; a journal of technical methods and pathology 2009 Jan;89(1):15-25

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Induction of an epithelial integrin alphavbeta6 in human cytomegalovirus-infected endothelial cells leads to activation of transforming growth factor-beta1 and increased collagen production.

Tabata T, Kawakatsu H, Maidji E, Sakai T, Sakai K, Fang-Hoover J, Aiba M, Sheppard D, Pereira L
The American journal of pathology 2008 Apr;172(4):1127-40

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Activin receptor-like kinase 1 inhibits human microvascular endothelial cell migration: potential roles for JNK and ERK.

David L, Mallet C, Vailhé B, Lamouille S, Feige JJ, Bailly S
Journal of cellular physiology 2007 Nov;213(2):484-9

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Cyclic stretch controls the expression of CD40 in endothelial cells by changing their transforming growth factor-beta1 response.

Korff T, Aufgebauer K, Hecker M
Circulation 2007 Nov 13;116(20):2288-97

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Identification of BMP9 and BMP10 as functional activators of the orphan activin receptor-like kinase 1 (ALK1) in endothelial cells.

David L, Mallet C, Mazerbourg S, Feige JJ, Bailly S
Blood 2007 Mar 1;109(5):1953-61

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The transforming growth factor-beta/Smad2,3 signalling axis is impaired in experimental pulmonary hypertension.

Zakrzewicz A, Kouri FM, Nejman B, Kwapiszewska G, Hecker M, Sandu R, Dony E, Seeger W, Schermuly RT, Eickelberg O, Morty RE
The European respiratory journal 2007 Jun;29(6):1094-104