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LearnH00007273-M09
antibody from Abnova Corporation
Targeting: TTN
CMD1G, CMH9, CMPD4, FLJ32040, LGMD2J, MYLK5, TMD
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00007273-M09 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00007273-M09, RRID:AB_607253
- Product name
- TTN monoclonal antibody (M09), clone 6H5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant TTN.
- Antigen sequence
MTTQAPTFTQPLQSVVVLEGSTATFEAHISGFPVP
EVSWFRDGQVISTSTLPGVQISFSDGRAKLTIPAV
TKANSGRYSLKATNGSGQATSTAELLVKAETAPPN
FVQRL- Isotype
- IgG
- Antibody clone number
- 6H5
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of TTN expression in transfected 293T cell line by TTN monoclonal antibody (M09), clone 6H5.Lane 1: TTN transfected lysate(32.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged TTN is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to TTN on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to TTN on formalin-fixed paraffin-embedded human heart. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
