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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- R30852 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- SIP Antibody
- Antibody type
- Polyclonal
- Antigen
- An amino acid sequence from the middle region of human SIAH Interacting Protein (NTRWDYLTQVEKECKE) was used as the immunogen for this SIP antibody (100% homologous in human, mouse and rat).
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the SIP antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of SIP antibody and Lane 1: rat liver ; 2: (r) brain; 3: (r) spleen; 4: human SMMC-7721; 5: (h) COLO320; 6: (h) SW620; 7: (h) 293T cell lysate
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC: A549 cells
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human liver cancer tissue with SIP antibody. HIER: boil sections in pH6 citrate buffer for 20 min and allow to cool before staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE rat brain with SIP antibody. HIER: boil sections in pH6 citrate buffer for 20 min and allow to cool before staining.
