Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AM05641PU-N - Provider product page
- Provider
- OriGene
- Product name
- Hexokinase 1 (HK1) mouse monoclonal antibody, clone 4D7, Purified
- Antibody type
- Monoclonal
- Description
- Hexokinase 1 (HK1) mouse monoclonal antibody, clone 4D7, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HK1
- Isotype
- IgG
- Antibody clone number
- 4D7
- Vial size
- 100 µg
- Concentration
- 1.0 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis: (A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase (1:1000). (B):Cell lysates (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- (A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase (1:1000). (B): Cell lysates (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence of human HeLa cells stained with Hoechst 33342 (Blue) and monoclonal anti-Hexokinase antibody (1:1000) with Alexa 488 (Green).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry: HK1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Brain, Cortex at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
- Validation comment
- IHC
