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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- AM31798PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM31798PU-N, RRID:AB_10976741
- Product name
- anti Hexokinase-1
- Antibody type
- Monoclonal
- Antigen
- HK1 antibody was raised against hK1 (NP_277031, 101 a.a. ~ 201 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 5G9
- Vial size
- 50 µg
- Concentration
- 0,36 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot analysis of HK1 expression in K-562 cell lysate.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot analysis of HK1 expression in transfected 293T cell line by HK1 monoclonal antibody.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of HK1 over-expressed 293 cell line, cotransfected with HK1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with HK1 monoclonal antibody. GAPDH (36.1 kDa) used as control.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged HK1 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunoprecipitation of HK1 transfected lysate using anti-HK1 monoclonal antibody and Protein A Magnetic Bead.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Human Small Intestine, Submucosal Plexus: Formalin-Fixed, Paraffin-Embedded (FFPE)
