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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-31143 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Tenascin R Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: mouse brain.
- Concentration
- 0.64 mg/mL
Submitted references Dietary palmitic acid promotes a prometastatic memory via Schwann cells.
Pascual G, Domínguez D, Elosúa-Bayes M, Beckedorff F, Laudanna C, Bigas C, Douillet D, Greco C, Symeonidi A, Hernández I, Gil SR, Prats N, Bescós C, Shiekhattar R, Amit M, Heyn H, Shilatifard A, Benitah SA
Nature 2021 Nov;599(7885):485-490
Nature 2021 Nov;599(7885):485-490
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TNR using anti-TNR Polyclonal Antibody (PA5-31143), in Mouse Brain (50 ug of cell lysate) and at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Tenascin R was performed by separating 50 µg of mouse tissue extract by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Tenascin R Polyclonal Antibody (Product # PA5-31143) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Tenascin R was performed in DIV9 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Tenascin R Polyclonal Antibody (Product # PA5 31143) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
