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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AF2910 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Alkaline Phosphatase/ALPL Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Alkaline Phosphatase/ALPL in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 60% cross-reactivity with recombinant human ALPL is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Optimal stimulation toward the dermal papilla lineage can be promoted by combined use of osteogenic and adipogenic inducers.
The Human Amniotic Mesenchymal Stem Cells (hAMSCs) Improve the Implant Osseointegration and Bone Regeneration in Maxillary Sinus Floor Elevation in Rabbits.
Responses of primary osteoblasts and osteoclasts from hemizygous β-globin knockout thalassemic mice with elevated plasma glucose to 1,25-dihydroxyvitamin D(3).
Mesenchymal Precursor Cells in Adult Nerves Contribute to Mammalian Tissue Repair and Regeneration.
Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia.
Multicolor quantitative confocal imaging cytometry.
Morphological and functional analyses of skeletal muscles from an immunodeficient animal model of limb-girdle muscular dystrophy type 2E.
Tissue-Nonspecific Alkaline Phosphatase Is Required for MC3T3 Osteoblast-Mediated Protection of Acute Myeloid Leukemia Cells from Apoptosis.
Cardiac Fibroblasts Adopt Osteogenic Fates and Can Be Targeted to Attenuate Pathological Heart Calcification.
Ablation of coactivator Med1 switches the cell fate of dental epithelia to that generating hair.
DLX3 regulates bone mass by targeting genes supporting osteoblast differentiation and mineral homeostasis in vivo.
Midkine-deficiency increases the anabolic response of cortical bone to mechanical loading.
Kazi T, Niibe I, Nishikawa A, Matsuzaki T
FEBS open bio 2020 Feb;10(2):197-210
FEBS open bio 2020 Feb;10(2):197-210
The Human Amniotic Mesenchymal Stem Cells (hAMSCs) Improve the Implant Osseointegration and Bone Regeneration in Maxillary Sinus Floor Elevation in Rabbits.
Yin L, Zhou ZX, Shen M, Chen N, Jiang F, Wang SL
Stem cells international 2019;2019:9845497
Stem cells international 2019;2019:9845497
Responses of primary osteoblasts and osteoclasts from hemizygous β-globin knockout thalassemic mice with elevated plasma glucose to 1,25-dihydroxyvitamin D(3).
Charoenphandhu N, Aeimlapa R, Sooksawanwit S, Thongbunchoo J, Teerapornpuntakit J, Svasti S, Wongdee K
Scientific reports 2019 Sep 27;9(1):13963
Scientific reports 2019 Sep 27;9(1):13963
Mesenchymal Precursor Cells in Adult Nerves Contribute to Mammalian Tissue Repair and Regeneration.
Carr MJ, Toma JS, Johnston APW, Steadman PE, Yuzwa SA, Mahmud N, Frankland PW, Kaplan DR, Miller FD
Cell stem cell 2019 Feb 7;24(2):240-256.e9
Cell stem cell 2019 Feb 7;24(2):240-256.e9
Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia.
Liu W, Zhang L, Xuan K, Hu C, Li L, Zhang Y, Jin F, Jin Y
Theranostics 2018;8(20):5575-5592
Theranostics 2018;8(20):5575-5592
Multicolor quantitative confocal imaging cytometry.
Coutu DL, Kokkaliaris KD, Kunz L, Schroeder T
Nature methods 2018 Jan;15(1):39-46
Nature methods 2018 Jan;15(1):39-46
Morphological and functional analyses of skeletal muscles from an immunodeficient animal model of limb-girdle muscular dystrophy type 2E.
Giovannelli G, Giacomazzi G, Grosemans H, Sampaolesi M
Muscle & nerve 2018 Feb 24;
Muscle & nerve 2018 Feb 24;
Tissue-Nonspecific Alkaline Phosphatase Is Required for MC3T3 Osteoblast-Mediated Protection of Acute Myeloid Leukemia Cells from Apoptosis.
Sterner RM, Kremer KN, Dudakovic A, Westendorf JJ, van Wijnen AJ, Hedin KE
Journal of immunology (Baltimore, Md. : 1950) 2018 Aug 1;201(3):1086-1096
Journal of immunology (Baltimore, Md. : 1950) 2018 Aug 1;201(3):1086-1096
Cardiac Fibroblasts Adopt Osteogenic Fates and Can Be Targeted to Attenuate Pathological Heart Calcification.
Pillai ICL, Li S, Romay M, Lam L, Lu Y, Huang J, Dillard N, Zemanova M, Rubbi L, Wang Y, Lee J, Xia M, Liang O, Xie YH, Pellegrini M, Lusis AJ, Deb A
Cell stem cell 2017 Feb 2;20(2):218-232.e5
Cell stem cell 2017 Feb 2;20(2):218-232.e5
Ablation of coactivator Med1 switches the cell fate of dental epithelia to that generating hair.
Yoshizaki K, Hu L, Nguyen T, Sakai K, He B, Fong C, Yamada Y, Bikle DD, Oda Y
PloS one 2014;9(6):e99991
PloS one 2014;9(6):e99991
DLX3 regulates bone mass by targeting genes supporting osteoblast differentiation and mineral homeostasis in vivo.
Isaac J, Erthal J, Gordon J, Duverger O, Sun HW, Lichtler AC, Stein GS, Lian JB, Morasso MI
Cell death and differentiation 2014 Sep;21(9):1365-76
Cell death and differentiation 2014 Sep;21(9):1365-76
Midkine-deficiency increases the anabolic response of cortical bone to mechanical loading.
Liedert A, Mattausch L, Röntgen V, Blakytny R, Vogele D, Pahl M, Bindl R, Neunaber C, Schinke T, Harroch S, Amling M, Ignatius A
Bone 2011 Apr 1;48(4):945-51
Bone 2011 Apr 1;48(4):945-51
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse Alkaline Phosphatase/ALPL by Western Blot. Western blot shows lysates of D3 mouse embryonic stem cell line, MEF mouse embryonic feeder cells, and mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for Alkaline Phosphatase/ALPL at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Alkaline Phosphatase/ALPL in Mouse Embryo. Alkaline Phosphatase/ALPL was detected in immersion fixed frozen sections of mouse embryonic (E13.5) developing vertebra using Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF2910) at 2 µg/mL overnight at 4 °C. Tissue was stained red and counterstained with DAPI (blue). Image courtesy of Paul J. Simmons, Ph.D., Director, Center for Stem Cell Research, The University of Texas Health Science Center at Houston, Houston, Texas, USA. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of ALPL in Rat Stem Cells by Flow Cytometry. Rat Mesenchymal Stem Cells were stained with Goat Anti-Mouse Alkaline Phosphatase/ALPL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2910, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
