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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA324563 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal ALPL Antibody (Center)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal ALPL Antibody (Center)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- ALPL
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 2.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of ALPL (arrow) using rabbit polyclonal ALPL Antibody (Center) (Cat.#TA324563). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ALPL gene.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ALPL Antibody (Center) (Cat.#TA324563) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the ALPL antibody detected the ALPL protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of ALPL Antibody (Center)(Cat#TA324563) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ALPL Antibody (Center) (Cat. #TA324563)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ALPL Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ALPL Antibody (Center) (Cat. #TA324563) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
