MA5-24845

antibody from Invitrogen Antibodies

Targeting: ALPL HOPS, TNALP, TNAP, TNSALP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24845

Provider

Invitrogen Antibodies

Product name

ALPL Recombinant Rabbit Monoclonal Antibody (3G2)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

3G2

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

A novel lineage of osteoprogenitor cells with dual epithelial and mesenchymal properties govern maxillofacial bone homeostasis and regeneration after MSFL.

Weng Y, Wang H, Wu D, Xu S, Chen X, Huang J, Feng Y, Li L, Wang Z
Cell research 2022 Sep;32(9):814-830

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ALPL in Lane 1: HeLa whole cell lysate, Lane 2: HepG2 whole cell lysate, Lane 3: MG-63 whole cell lysate. Samples were incubated with ALPL monoclonal antibody (Product # MA5-24845) at a dilution of 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of ALPL in SW480 cells (green). Samples were treated with paraformaldehyde, permeabilized with 0.25% Triton and PBS and incubated with ALPL monoclonal antibody (Product # MA5-24845).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of ALPL in HepG2 cells (green). Samples were treated with paraformaldehyde, permeabilized with 0.25% Triton and PBS, and incubated with ALPL monoclonal antibody (Product # MA5-24845).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of ALPL in HeLa cells (green). Samples were treated with paraformaldehyde, permeabilized with 0.25% Triton and PBS, and incubated with ALPL monoclonal antibody (Product # MA5-24845).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ALPL in paraffin-embedded mouse small intestine tissue. Samples were incubated with ALPL monoclonal antibody (Product # MA5-24845) followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ALPL in paraffin-embedded mouse small intestine tissue. Samples were incubated with ALPL monoclonal antibody (Product # MA5-24845) followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ALPL in paraffin-embedded mouse kidney tissue. Samples were incubated with ALPL monoclonal antibody (Product # MA5-24845) followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ALPL in paraffin-embedded human liver tissue. Samples were incubated with ALPL monoclonal antibody (Product # MA5-24845) followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ALPL in paraffin-embedded human liver cancer tissue. Samples were incubated with ALPL monoclonal antibody (Product # MA5-24845) followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of ALPL in paraffin-embedded human kidney tissue. Samples were incubated with ALPL monoclonal antibody (Product # MA5-24845) followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of ALPL in HeLa cells (blue) and compared with an unlabeled control (cells without incubation with primary antibody; red). Samples were incubated with ALPL monoclonal antibody (Product # MA5-24845) at a dilution of 1:50 followed by Alexa Fluor 488-conjugated goat anti rabbit IgG.