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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00023179-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00023179-M01, RRID:AB_2269551
- Product name
- Mouse Monoclonal RGL1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RGL1 - ral guanine nucleotide dissociation stimulator-like 1
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references The anti-inflammatory TIPE2 is an inhibitor of the oncogenic Ras.
Gus-Brautbar Y, Johnson D, Zhang L, Sun H, Wang P, Zhang S, Zhang L, Chen YH
Molecular cell 2012 Mar 9;45(5):610-8
Molecular cell 2012 Mar 9;45(5):610-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RGL1 Antibody (2D10) [H00023179-M01] - Analysis of RGL1 expression in transfected 293T cell line by RGL1 monoclonal antibody (M01), clone 2D10.Lane 1: RGL1 transfected lysate(90.7 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RGL1 Antibody (2D10) [H00023179-M01] - RGL1 monoclonal antibody (M01), clone 2D10 Analysis of RGL1 expression in A-431.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: RGL1 Antibody (2D10) [H00023179-M01] - Detection limit for recombinant GST tagged RGL1 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RGL1 Antibody (2D10) [H00023179-M01] - Analysis of monoclonal antibody to RGL1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
