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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- GTX48486 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX48486, RRID:AB_11170083
- Product name
- RNF2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian
- Host
- Goat
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot using GeneTex's Affinity Purified anti-RING1B antibody shows detection of a 38 kDa band corresponding to human RING1B in 3T3 (lane 1), U937 (lane 2), Jurkat (lane 3), mouse brain (lane 4) and CHO-K1 (lane 5) cell lysates. Approximately 20 µg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-RING1B antibody incubated at room temper-ature. Signal was detected using standard techniques.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot using GeneTex's Affinity Purified anti-RING1B antibody shows detection of a 38 kDa band corresponding to human RING1B in 3T3 (lane 1), U937 (lane 2), Jurkat (lane 3), mouse brain (lane 4) and CHO-K1 (lane 5) cell lysates. Approximately 20 ?g of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-RING1B antibody incubated at room temperature. Signal was detected using standard techniques.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence Microscopy of Goat anti-RING1B antibody (GTX48486). Tissue: human HeLa cells. Fixation: methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25¢XC. Antigen retrieval: not required. Primary antibody: RING1B antibody at 1:300 for 20 minutes at 25¢XC. Secondary antibody: Alexa Fluor?488-conjugated Donkey anti-goat IgG secondary antibody at 1:500 for 45 min at RT. Localization: RING1B is nuclear and occasionally cytoplasmic. Staining: RING1B (RNF2) as green signal, Tubulin cytoplasm staining red, and DAPI (blue) nuclear counterstain.
