H00006045-M01
antibody from Novus Biologicals
Targeting: RNF2
BAP-1, BAP1, DING, HIPI3, RING1B, RING2
Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [4]
- ELISA [1]
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Validation data
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- Product number
- H00006045-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006045-M01, RRID:AB_547778
- Product name
- Mouse Monoclonal RNF2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RNF2 - ring finger protein 2
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Scmh1 has E3 ubiquitin ligase activity for geminin and histone H2A and regulates geminin stability directly or indirectly via transcriptional repression of Hoxa9 and Hoxb4.
Direct role of Bardet-Biedl syndrome proteins in transcriptional regulation.
Distinct histone modifications in stem cell lines and tissue lineages from the early mouse embryo.
Polycomb-group complex 1 acts as an E3 ubiquitin ligase for Geminin to sustain hematopoietic stem cell activity.
Yasunaga S, Ohtsubo M, Ohno Y, Saeki K, Kurogi T, Tanaka-Okamoto M, Ishizaki H, Shirai M, Mihara K, Brock HW, Miyoshi J, Takihara Y
Molecular and cellular biology 2013 Feb;33(4):644-60
Molecular and cellular biology 2013 Feb;33(4):644-60
Direct role of Bardet-Biedl syndrome proteins in transcriptional regulation.
Gascue C, Tan PL, Cardenas-Rodriguez M, Libisch G, Fernandez-Calero T, Liu YP, Astrada S, Robello C, Naya H, Katsanis N, Badano JL
Journal of cell science 2012 Jan 15;125(Pt 2):362-75
Journal of cell science 2012 Jan 15;125(Pt 2):362-75
Distinct histone modifications in stem cell lines and tissue lineages from the early mouse embryo.
Rugg-Gunn PJ, Cox BJ, Ralston A, Rossant J
Proceedings of the National Academy of Sciences of the United States of America 2010 Jun 15;107(24):10783-90
Proceedings of the National Academy of Sciences of the United States of America 2010 Jun 15;107(24):10783-90
Polycomb-group complex 1 acts as an E3 ubiquitin ligase for Geminin to sustain hematopoietic stem cell activity.
Ohtsubo M, Yasunaga S, Ohno Y, Tsumura M, Okada S, Ishikawa N, Shirao K, Kikuchi A, Nishitani H, Kobayashi M, Takihara Y
Proceedings of the National Academy of Sciences of the United States of America 2008 Jul 29;105(30):10396-401
Proceedings of the National Academy of Sciences of the United States of America 2008 Jul 29;105(30):10396-401
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNF2 Antibody (6C2) [H00006045-M01] - Analysis of RNF2 over-expressed 293 cell line, cotransfected with RNF2 Validated Chimera RNAi ( Cat # H00006045-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RNF2 monoclonal antibody (M01), clone 6C2 (Cat # H00006045-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNF2 Antibody (6C2) [H00006045-M01] - Analysis of RNF2 expression in transfected 293T cell line by RNF2 monoclonal antibody (M01), clone 6C2.Lane 1: RNF2 transfected lysate(37.7 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNF2 Antibody (6C2) [H00006045-M01] - RNF2 monoclonal antibody (M01), clone 6C2. Analysis of RNF2 expression in Raw 264.7.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNF2 Antibody (6C2) [H00006045-M01] - RNF2 monoclonal antibody (M01), clone 6C2 Analysis of RNF2 expression in NIH/3T3.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: RNF2 Antibody (6C2) [H00006045-M01] - Detection limit for recombinant GST tagged RNF2 is approximately 0.03ng/ml as a capture antibody.