CABT-RM132

antibody from Creative Diagnostics

Targeting: RNF2 BAP-1, BAP1, DING, HIPI3, RING1B, RING2

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Chromatin Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

CABT-RM132

Provider

Creative Diagnostics

Product name

Mouse Anti-RNF2 monoclonal antibody, clone 4-4

Antibody type

Monoclonal

Description

E3 ubiquitin-protein ligase RING2 is encoded by the Rnf2 gene in murine species. RING2 is an essential component of Polycomb-repressive complex 1 (PRC1) and serves as the main E3 ubiquitin ligase and plays a role in the transcriptional repression of genes that are required for pluripotency in embryonic stem cells. PRC1 and PRC2 act to maintain repression at many developmental genes in mouse embryonic stem cells and are required for early development. PRC1 mediates Histone H2A mono-ubiquitination at K119 (H2AK119ub1) and PRC2 mediates H3 tri-methylation at K27 (H3K27me3). H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. RING2 is predominantly expressed in embryonic stem cells. It has a RING-type zinc-finger domain (aa 51-91). RING2 inactivation is reported to lead to phenotypes that combine both differentiation and proliferation defects that can lead to upregulation of tumor suppressors that inhibit cyclin-dependent kinases (CDKs) and control cell proliferation by modulating S-phase entry. RING2 null mutations lead to embryonic lethality.

Reactivity

Human, Mouse, Simian

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG