HPA009027

antibody from Atlas Antibodies

Targeting: ULK2 ATG1B, KIAA0623, Unc51.2

Western blot (Enhanced validation)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA009027

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA009027, RRID:AB_1080458

Product name

Anti-ULK2

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

VVRRSNTSPMGFLRPGSCSPVPADTAQTVGRRLST
GSSRPYSPSPLVGTIPEQFSQCCCGHPQGHDSRSR
NSSGSPVPQAQSPQSLLSGARLQSAPTLTDIYQNK
QKLRKQHSDPVCPSHTGAGYSYSPQPSRPGSLGTS
PTKH


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References

Submitted references

Transcriptional control of autophagy-lysosome function drives pancreatic cancer metabolism.

Perera RM, Stoykova S, Nicolay BN, Ross KN, Fitamant J, Boukhali M, Lengrand J, Deshpande V, Selig MK, Ferrone CR, Settleman J, Stephanopoulos G, Dyson NJ, Zoncu R, Ramaswamy S, Haas W, Bardeesy N
Nature 2015 Aug 20;524(7565):361-5

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Methylation silencing of ULK2, an autophagy gene, is essential for astrocyte transformation and tumor growth.

Shukla S, Patric IR, Patil V, Shwetha SD, Hegde AS, Chandramouli BA, Arivazhagan A, Santosh V, Somasundaram K
The Journal of biological chemistry 2014 Aug 8;289(32):22306-18

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and ULK2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402363).

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human urinary bladder shows cytoplasmic and membranous positivity in urothelial cells.

Sample type

HUMAN