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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- V3392 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Plakophilin 1 Antibody
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 10B2
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml
- Storage
- Store the Plakophilin 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Lack of plakoglobin leads to lethal congenital epidermolysis bullosa: a novel clinico-genetic entity.
Homozygous splice site mutations in PKP1 result in loss of epidermal plakophilin 1 expression and underlie ectodermal dysplasia/skin fragility syndrome in two consanguineous families.
Pigors M, Kiritsi D, Krümpelmann S, Wagner N, He Y, Podda M, Kohlhase J, Hausser I, Bruckner-Tuderman L, Has C
Human molecular genetics 2011 May 1;20(9):1811-9
Human molecular genetics 2011 May 1;20(9):1811-9
Homozygous splice site mutations in PKP1 result in loss of epidermal plakophilin 1 expression and underlie ectodermal dysplasia/skin fragility syndrome in two consanguineous families.
Sprecher E, Molho-Pessach V, Ingber A, Sagi E, Indelman M, Bergman R
The Journal of investigative dermatology 2004 Mar;122(3):647-51
The Journal of investigative dermatology 2004 Mar;122(3):647-51
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) U87, 2) HeLa and 3) A431 cell lysate with Plakophilin 1 antibody (clone 10B2). Expected molecular weight: 75-83 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free Plakophilin 1 Antibody (clone 10B2). Confirmation of Integrity and Purity of the Antibody.
