Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [4]
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Validation data
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- Product number
- NBP1-90042 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-90042, RRID:AB_11057301
- Product name
- Rabbit Polyclonal Plakophilin 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, rat Plakophilin 1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Aberrantly methylated PKP1 in the progression of Barrett's esophagus to esophageal adenocarcinoma.
Kaz AM, Luo Y, Dzieciatkowski S, Chak A, Willis JE, Upton MP, Leidner RS, Grady WM
Genes, chromosomes & cancer 2012 Apr;51(4):384-93
Genes, chromosomes & cancer 2012 Apr;51(4):384-93
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Plakophilin 1 Antibody [NBP1-90042] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Plakophilin 1 Antibody [NBP1-90042] - Analysis in human cell line PC-3.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Plakophilin 1 Antibody [NBP1-90042] - Analysis in human cell lines PC-3 and A-549 using anti-PKP1 antibody. Corresponding PKP1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Plakophilin 1 Antibody [NBP1-90042] - Analysis using Anti-PKP1 antibody NBP1-90042 (A) shows similar pattern to independent antibody NBP1-90041 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Plakophilin 1 Antibody [NBP1-90042] - Staining of human oral mucosa shows strong cytoplasmic, membranous and nuclear positivity in squamous epithelial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Plakophilin 1 Antibody [NBP1-90042] - Staining of human skeletal muscle shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Plakophilin 1 Antibody [NBP1-90042] - Staining of human skin shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Plakophilin 1 Antibody [NBP1-90042] - Staining in human skin and skeletal muscle tissues using anti-PKP1 antibody. Corresponding PKP1 RNA-seq data are presented for the same tissues.