AGR-050-25UL

antibody from Invitrogen Antibodies

Targeting: GPR37L1 ETBR-LP-2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AGR-050-25UL

Provider

Invitrogen Antibodies

Product name

GPR37L1 (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

25 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4): - 1-2. Anti-GPR37L1 (extracellular) Antibody (#AGR-050), (1:200). 3-4. Anti-GPR37L1 (extracellular) Antibody , preincubated with GPR37L1 (extracellular) Blocking Peptide (BLP-GR050).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human U-87 MG glioblastoma cell line lysate: - 1. Anti-GPR37L1 (extracellular) Antibody (#AGR-050), (1:200). 2. Anti-GPR37L1 (extracellular) Antibody , preincubated with GPR37L1 (extracellular) Blocking Peptide (BLP-GR050).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain membranes (lanes 2 and 4): - 1-2. Anti-GPR37L1 (extracellular) Antibody (#AGR-050), (1:200). 3-4. Anti-GPR37L1 (extracellular) Antibody , preincubated with GPR37L1 (extracellular) Blocking Peptide (BLP-GR050).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of GPR37L1 in rat cerebellum. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR37L1 (extracellular) Antibody (#AGR-050), (1:300), followed by goat Anti-rabbit-AlexaFluor-488. A. GPR37L1 immunoreactivity (green) appeared around Purkinje cell soma (arrows) and in the molecular layer (M). B. Pre-incubation of the Antibody with GPR37L1 (extracellular) Blocking Peptide (BLP-GR050), suppressed staining. Cell nuclei are stained with DAPI (blue).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cell surface detection of GPR37L1 by indirect flow cytometry in live intact mouse BV-2 microglia cell line: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-GPR37L1 (extracellular) Antibody (#AGR-050), (2.5 µg) + goat- Anti-rabbit-FITC.