A301-994A

antibody from Invitrogen Antibodies

Targeting: MAP3K20 MLK7, MLTK, MLTKalpha, MLTKbeta, MRK, ZAK

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-994A

Provider

Invitrogen Antibodies

Product name

ZAK Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human ZAK by immunohistochemistry. Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti- ZAK (Cat. No. A301-994A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human ZAK by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZAK antibody A301-994A used for IP at 3 µg/mg lysate. ZAK was also immunoprecipitated by rabbit anti-ZAK antibody A301-993A, which recognizes an upstream epitope. For blotting immunoprecipitated ZAK, A301-993A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.