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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- PAB14079 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB14079, RRID:AB_10554285
- Product name
- Jarid1b polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of Jarid1b.
- Storage
- Store at -20°C. For long term storage store at -80°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot was performed on whole cell lysates from mouse fibroblasts (Lane 1, NIH/3T3) and embryonic stem cells (Lane 2, E14Tg2a) with Jarid1b polyclonal antibody (Cat # PAB14079), diluted 1 : 500 in BSA/PBS-Tween.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA was performed using a serial dilution of Jarid1b polyclonal antibody (Cat # PAB14079). The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the crude serum was estimated to be 1 : 2,200.
- Validation comment
- ELISA
