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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Flow cytometry [2]
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- Product number
- NBP1-28635 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-28635, RRID:AB_1914103
- Product name
- Rabbit Polyclonal Dtk/TYRO3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.19 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Dengue viruses infect human megakaryocytes, with probable clinical consequences.
Platelets Promote Macrophage Polarization toward Pro-inflammatory Phenotype and Increase Survival of Septic Mice.
The TAM family receptor tyrosine kinase TYRO3 is a negative regulator of type 2 immunity.
Vogt MB, Lahon A, Arya RP, Spencer Clinton JL, Rico-Hesse R
PLoS neglected tropical diseases 2019 Nov;13(11):e0007837
PLoS neglected tropical diseases 2019 Nov;13(11):e0007837
Platelets Promote Macrophage Polarization toward Pro-inflammatory Phenotype and Increase Survival of Septic Mice.
Carestia A, Mena HA, Olexen CM, Ortiz Wilczyñski JM, Negrotto S, Errasti AE, Gómez RM, Jenne CN, Carrera Silva EA, Schattner M
Cell reports 2019 Jul 23;28(4):896-908.e5
Cell reports 2019 Jul 23;28(4):896-908.e5
The TAM family receptor tyrosine kinase TYRO3 is a negative regulator of type 2 immunity.
Chan PY, Carrera Silva EA, De Kouchkovsky D, Joannas LD, Hao L, Hu D, Huntsman S, Eng C, Licona-Limón P, Weinstein JS, Herbert DR, Craft JE, Flavell RA, Repetto S, Correale J, Burchard EG, Torgerson DG, Ghosh S, Rothlin CV
Science (New York, N.Y.) 2016 Apr 1;352(6281):99-103
Science (New York, N.Y.) 2016 Apr 1;352(6281):99-103
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Dtk/TYRO3 Antibody [NBP1-28635] - Lane 1: Western blot on normal human brain, Lane 2: Western blot on normal mouse brain.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): Dtk/TYRO3 Antibody [NBP1-28635] - An intracellular stain was performed on U-937 cells with Dtk/TYRO3 Antibody NBP1-28635AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Dtk/TYRO3 Antibody [NBP1-28635] - An intracellular stain was performed on HeLa cells with Dtk/TYRO3 Antibody NBP1-28635AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
