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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- TA505706 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA505706, RRID:AB_2623305
- Product name
- MDM4 (MDMX) mouse monoclonal antibody, clone OTI4B5 (formerly 4B5)
- Antibody type
- Monoclonal
- Description
- MDM4 (MDMX) mouse monoclonal antibody, clone OTI4B5 (formerly 4B5)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- MDM4
- Isotype
- IgG
- Antibody clone number
- OTI4B5
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
Submitted references MDM4/HIPK2/p53 cytoplasmic assembly uncovers coordinated repression of molecules with anti-apoptotic activity during early DNA damage response.
Mancini F, Pieroni L, Monteleone V, Lucà R, Fici L, Luca E, Urbani A, Xiong S, Soddu S, Masetti R, Lozano G, Pontecorvi A, Moretti F
Oncogene 2016 Jan 14;35(2):228-40
Oncogene 2016 Jan 14;35(2):228-40
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MDM4 (RC209620, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MDM4.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-MDM4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-MDM4 mouse monoclonal antibody (TA505706) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MDM4(RC209620).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MDM4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505706)
- Validation comment
- IHC
