A700-000

antibody from Invitrogen Antibodies

Targeting: MDM4 HDMX, MDMX

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A700-000

Provider

Invitrogen Antibodies

Product name

HdmX/MDM4 Recombinant Rabbit Monoclonal Antibody (BL-3-2F2)

Antibody type

Monoclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

BL-3-2F2

Vial size

100 µL

Concentration

0.10 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human HdmX/MDM4 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, Jurkat, GaMG, A-549, Hep-G2, U2OS, RKO, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-HdmX/MDM4 recombinant monoclonal antibody [BL-3-2F2] (Product # A700-000 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human HdmX/MDM4 by WB of immunoprecipitates from Jurkat lysate. Antibodies: Rabbit anti-HdmX/MDM4 recombinant monoclonal [BL-3-2F2] (A700-000) and rabbit anti-HdmX/MDM4 (BL15229). Secondary: ReliaBLOT® reagents (WB120).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human HdmX/MDM4 by WB of Jurkat, 293T, HeLa, and A549 lysate Antibody: Rabbit anti-HdmX/MDM4 recombinant monoclonal [BL-3-2F2] (A700-000). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human HdmX/MDM4 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-HdmX/MDM4 recombinant monoclonal [BL-3-2F2] (Product #A700-000; lot 3) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG (Product # A120-101D2).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human HdmX/MDM4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-HdmX/MDM4 recombinant monoclonal antibody [BL-3-2F2] (Product # A700-000 lot 3) used for IP at 20 µL/mg lysate. HdmX/MDM4 was also immunoprecipitated by rabbit anti-HdmX/MDM4 antibody (Product # A300-287A). For blotting immunoprecipitated HdmX/MDM4 (Product # A700-000) was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.