Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- A700-000 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HdmX/MDM4 Recombinant Rabbit Monoclonal Antibody (BL-3-2F2)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- BL-3-2F2
- Vial size
- 100 µL
- Concentration
- 0.10 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human HdmX/MDM4 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, Jurkat, GaMG, A-549, Hep-G2, U2OS, RKO, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-HdmX/MDM4 recombinant monoclonal antibody [BL-3-2F2] (Product # A700-000 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human HdmX/MDM4 by WB of immunoprecipitates from Jurkat lysate. Antibodies: Rabbit anti-HdmX/MDM4 recombinant monoclonal [BL-3-2F2] (A700-000) and rabbit anti-HdmX/MDM4 (BL15229). Secondary: ReliaBLOT® reagents (WB120).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human HdmX/MDM4 by WB of Jurkat, 293T, HeLa, and A549 lysate Antibody: Rabbit anti-HdmX/MDM4 recombinant monoclonal [BL-3-2F2] (A700-000). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human HdmX/MDM4 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-HdmX/MDM4 recombinant monoclonal [BL-3-2F2] (Product #A700-000; lot 3) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG (Product # A120-101D2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human HdmX/MDM4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-HdmX/MDM4 recombinant monoclonal antibody [BL-3-2F2] (Product # A700-000 lot 3) used for IP at 20 µL/mg lysate. HdmX/MDM4 was also immunoprecipitated by rabbit anti-HdmX/MDM4 antibody (Product # A300-287A). For blotting immunoprecipitated HdmX/MDM4 (Product # A700-000) was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.