Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-39143 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-TESK1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Regulation of cofilin phosphorylation in glomerular podocytes by testis specific kinase 1 (TESK1).
Wang L, Buckley AF, Spurney RF
Scientific reports 2018 Aug 16;8(1):12286
Scientific reports 2018 Aug 16;8(1):12286
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of TESK1 in extracts from rat heart cells, rat kidney cells and rat liver cells using a TESK1 polyclonal antibody (Product # PA5-39143).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Expression of TESK1 in glomerular podocytes. ( a ) PCR products of the appropriate size were amplified from human (Hu) and mouse (Ms) podocytes using intron spanning primers. DNA sequencing confirmed amplification of TESK1. (Supplementary Figure S1 ) ( b ) A monoclonal antibody to Hu TESK1 (#1) and polyclonal antibody to TESK1 (#2) confirmed expression of TESK1 in Hu podocytes. The antibody did not react with Ms TESK1. ( c ) We next investigated TESK1 expression in Hu kidney sections using the monoclonal antibody to TESK1 (green) and the podocyte marker synaptopodin (SYN) (red) as described in the Methods Section. Nuclei were counterstained with DAPI (blue). Merging of the images shows that TESK1 co-localized with the podocyte marker SYN at focal subcellular locations within the cell (insets). TESK1 was also found in tubular cells. TESK1 and SYN staining were not detected in the absence of the primary antibodies. ( d ) To determine if podocytes bound fibronectin, we performed attachment assays using fibronectin and collagen coated tissue dishes in the presence or absence of the integrin beta3 inhibitor peptide cyclo-RGDfV. Mouse podocyte bound avidly to fibronectin and the binding was inhibited by cyclo-RGDfV. Podocytes bound collagen less avidly and cylco-RGDfV did not significantly affect binding to collagen-coated dishes. ( e , f ) Plating podocytes on fibronectin decreased CFL1 phosphorylation compared to cells plated on collagen. ( g , h ) Rho A activity was enh