AF2056

antibody from R&D Systems

Targeting: RPS6KA1 HU-1, MAPKAPK1, p90Rsk, RSK1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2056

Provider

R&D Systems

Product name

Human/Mouse/Rat RSK Pan Specific Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects endogenous human, mouse and rat RSK family isoforms in Western blots.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Integrins promote cytokinesis through the RSK signaling axis.

Mathew SS, Nieves B, Sequeira S, Sambandamoorthy S, Pumiglia K, Larsen M, Laflamme SE
Journal of cell science 2014 Feb 1;127(Pt 3):534-45

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human and Mouse RSK by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, KG-1 human acute myelogenous leukemia cell line, TS1 mouse helper T cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat RSK Pan Specific, Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2056) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for RSK Pan Specific, at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.