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Learn600-401-B30
antibody from Rockland Immunochemicals, Inc.
Targeting: RPS6KA1
HU-1, MAPKAPK1, p90Rsk, RSK1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 600-401-B30 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-B30, RRID:AB_1961748
- Product name
- Anti-p90 RSK1 pS732 (RABBIT) Antibody - 600-401-B30
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references Interactions between the regulatory subunit of type I protein kinase A and p90 ribosomal S6 kinase1 regulate cardiomyocyte apoptosis.
Gao X, Lin B, Sadayappan S, Patel TB
Molecular pharmacology 2014 Feb;85(2):357-67
Molecular pharmacology 2014 Feb;85(2):357-67
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's affinity purified anti-p90 RSK1 pS732 antibody shows detection of a band ~90 kDa in size corresponding to phosphorylated p90 RSK1 (arrowhead) in EGF stimulated (lane 2) HEK293T cell lysates prepared from cells grown in the absence of serum for 12 h. No staining is observed in similarly prepared lysates derived from unstimulated (control) cells (lane 1). After transfer, the membrane was blocked overnight followed by reaction with the primary antibody at a 1:1,000 dilution. Detection occurred using a peroxidase conjugated secondary antibody and ECL. Personal Communication. Kuldeep Patel, Loyola University Medical Center, Maywood, IL.
- Validation comment
- Western Blot
