17170-1-AP

antibody from Invitrogen Antibodies

Targeting: IARS2 FLJ10326

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

17170-1-AP

Provider

Invitrogen Antibodies

Product name

IARS2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: KMSKSLGNV IHPDVVVNGG QDQSKEPPYG ADVLRWWVAD SNVFTEVAIG PSVLNAARDD ISKLRNTLRF LLGNVADFNP ETDSIPVNDM YVIDQYMLHL LQDLANKITE LYKQYDFGKV VRLLRTFYTR ELSNFYFSII KDRLYCEKEN DPKRRSCQTA LVEILDVIVR SFAPILPHLA EEVFQHIPYI KEPKSVFRTG WISTSSIWKK PGLEEAVESA CAMRDSFLGS IPGKNAAEYK VITVIEPGLL FEIIEMLQSE ETSSTSQLNE LMMASESTLL AQEPREMTAD VIELKGKFLI NLEGGDIREE SSYKVIVMPT TKEKCPRCWK YTAESSDTLC PRCAEVVSGK (425-773 aa encoded by BC010218)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.3 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse lung tissue were subjected to SDS PAGE followed by western blot with 17170-1-AP (IARS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 17170-1-AP (IARS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 17170-1-AP (IARS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using IARS2 antibody 17170-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 17170-1-AP (IARS2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 17170-1-AP (IARS2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 17170-1-AP (IARS2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 17170-1-AP (IARS2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-IARS2 (IP:17170-1-AP, 4ug; Detection:17170-1-AP 1:800) with HeLa cells lysate 4000ug.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-IARS2 (IP:17170-1-AP, 4 ug; Detection:17170-1-AP 1:800) with Jurkat cells lysate 4000 ug.