Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [4]
- Other assay [3]
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Validation data
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- Product number
- PA5-17494 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Calpain 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 54 µg/mL
- Storage
- -20°C
Submitted references Ca(2+) and calpain mediate capsaicin-induced ablation of axonal terminals expressing transient receptor potential vanilloid 1.
Distinct roles for μ-calpain and m-calpain in synaptic NMDAR-mediated neuroprotection and extrasynaptic NMDAR-mediated neurodegeneration.
Wang S, Wang S, Asgar J, Joseph J, Ro JY, Wei F, Campbell JN, Chung MK
The Journal of biological chemistry 2017 May 19;292(20):8291-8303
The Journal of biological chemistry 2017 May 19;292(20):8291-8303
Distinct roles for μ-calpain and m-calpain in synaptic NMDAR-mediated neuroprotection and extrasynaptic NMDAR-mediated neurodegeneration.
Wang Y, Briz V, Chishti A, Bi X, Baudry M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Nov 27;33(48):18880-92
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Nov 27;33(48):18880-92
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Calpain 2 Large Subunit (M-type) in extracts from C2C12 and PC3 cells using Calpain 2 Large Subunit (M-type) polyclonal antibody (Product # PA5-17494).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Calpain 2 (CAPN2) was achieved by transfecting A-431 with Calpain 2 (CAPN2) specific siRNAs (Silencer® select Products # s321). Western blot analysis (Fig. a) was performed using whole cell extracts from the Calpain 2 (CAPN2) knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with Calpain 2 Polyclonal Antibody (Product # PA5-17494, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to CAPN2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-Calpain 2 Polyclonal Antibody (Product # PA5-17494) and a 76 kDa band corresponding to Calpain 2 was observed in C2C12, DU 145, NIH/3T3, A-431 and PC-3, but not in Jurkat and Daudi which are reported to be negative. Whole cell lysates (30 µg lysate) of Jurkat (Lane 1), C2C12 (Lane 2), DU 145 (Lane 3), NIH/3T3 (Lane 4), A-431 (Lane 5), PC-3 (Lane 6) and Daudi (Lane 7) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Calpain-2 was performed by loading 20 µg of WT (lane 1) and CAPN2 CRISPR KO (lane 2) MDA-MB-231 cell lysates in RIPA buffer onto a 4-15% gradient polyacrylamide gel. Proteins on the blots were visualized with Ponceau staining (below immunoblot). Proteins were transferred to nitrocellulose membrane and blocked in 5% milk for 1 hr. CAPN2 was detected at approximately 74 kDa using a CAPN2 polyclonal antibody (Product # PA5-17494) at a dilution of 1:1,000 in 5% BSA in TBS with 0.1% Tween 20 (TBST) overnight at 4°C. The peroxidase-conjugated secondary antibody (Product # 65-6120) was diluted to 0.2 µg/mL in TBST with 5% milk for 1 hr. Chemiluminescent detection was performed using Pierce ECL Western Blotting Substrate (Product # 32106). Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL