Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-42741 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DEGS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Single Mutation in the NFU1 Gene Metabolically Reprograms Pulmonary Artery Smooth Muscle Cells.
Cell metabolism regulates integrin mechanosensing via an SLC3A2-dependent sphingolipid biosynthesis pathway.
James J, Zemskova M, Eccles CA, Varghese MV, Niihori M, Barker NK, Luo M, Mandarino LJ, Langlais PR, Rafikova O, Rafikov R
Arteriosclerosis, thrombosis, and vascular biology 2021 Feb;41(2):734-754
Arteriosclerosis, thrombosis, and vascular biology 2021 Feb;41(2):734-754
Cell metabolism regulates integrin mechanosensing via an SLC3A2-dependent sphingolipid biosynthesis pathway.
Boulter E, Estrach S, Tissot FS, Hennrich ML, Tosello L, Cailleteau L, de la Ballina LR, Pisano S, Gavin AC, FĂ©ral CC
Nature communications 2018 Nov 19;9(1):4862
Nature communications 2018 Nov 19;9(1):4862
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human muscle cells using an anti-DEGS1 polyclonal antibody (Product # PA5-42741).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Skeletal Muscle (Lane 1), Rat Skeletal Muscle (Lane 2), Mouse Skin (Lane 3) and Rat Eye (Lane 4). The blot was probed with Anti-DEGS1 Polyclonal Antibody (Product # PA5-42741, 0.4ug/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 ug/ml, 1:4000 dilution). A 38 kDa band corresponding to DEGS1 was observed tissues tested.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (paraffin-embedded) analysis of human lung tissue using an anti-DEGS1 polyclonal antibody (Product # PA5-42741).; Secondary Antibody: Donkey anti-Rabbit-Cy3.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 CD98hc determines the level of DES2 in fibroblasts. a - d Loss of CD98hc or expression of C330S mutant reduce the expression of DES2 and induces DES1. One experiment representative of at least n = 3 experiments. e - h Levels of DES1 and DES2 mRNA as measured by RT-qPCR on total mRNA from CD98hc- cells or C330S reconstituted cells. Means are plotted with s.e.m. as error bars from, respectively, n = 6 or n = 3 experiments. Differences were not statistically different in a Student's t test. i Schematic representation of the de novo sphingolipid biosynthesis pathway presenting variations of metabolites. Numbers refer to metabolites depicted and numbered in the volcano plot of Fig. 4d . Metabolites and p values are reported in Supplementary Data 1 . P values were calculated using a Student's t test. DES1/2 variations were extracted from results presented in Fig. 5a-d and Supplementary Figure 5A-D . j , k Hsp90 inhibition in control cells reduces the level of DES2 expressed in cells. Right panel, means are plotted with s.e.m. as error bars from n = 3 experiments, ** P < 0.01 in a Student's t test. l Grp78 and Grp94 co-immunoprecipitate with DES2 upon loss of CD98hc or geldanamycin treatment. Control, CD98hc-, or C330S-expressing cells were treated with geldanamycin for 16 h prior to cell lysis and DES2 immunoprecipitation. Precipitates were loaded on SDS-PAGE and analyzed by Western blot. One experiment representative of n = 2