Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- LS-B2540 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-B2540, RRID:AB_2135790
- Product name
- IHC-plus™ TGFB4 / LEFTY2 Antibody (clone 7C5G1H6H10) LS-B2540
- Antibody type
- Monoclonal
- Description
- Protein A purified
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 7C5G1H6H10
- Storage
- Store at 4°C or -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Mab anti-Human LEFTY antibody (clone 7C5G1H6H10) is shown to detect by western blot partially purified recombinant 6X His tagged human LEFTY. 1.0 mg of protein of protein was loaded per lane. The blot was incubated with a 1:2000 dilution of Mab anti-Human LEFTY.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Mab anti-Human LEFTY antibody (clone 7C5G1H6H10) is shown to detect by western blot partially purified recombinant 6X His tagged human LEFTY. Detection occurs after 1.0 ug of protein is loaded in each lane. The blot was incubated with a 1:2000 dilution of Mab anti-Human LEFTY at room temperature for 30 min followed by detection using IRDye800 labeled Goat-a-Mouse IgG [H&L] (610-132-121) diluted 1:1000. Lane 1 contains an unrelated 6X His tagged protein and shows that the antibody does not recognize the epitope tag. Lane 2 contains partially purified recombinant human LEFTY. The antibody may be used to detect endogenous human LEFTY. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.