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- Antibody Data
- Antigen structure
- References [19]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- 21645-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#21645-1-AP, RRID:AB_10858624
- Product name
- ROCK2(middle) antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated ROCK2(middle) antibody (Cat. #21645-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Therapeutic potential of Lingjiao Gouteng decoction in acute alcohol intoxication and alcohol-induced brain injury involving the RhoA/ROCK2/NF-κB signaling pathway.
Fasudil attenuates oxidative stress-induced partial epithelial-mesenchymal transition of tubular epithelial cells in hyperuricemic nephropathy via activating Nrf2.
ROCK2-RNA interaction map reveals multiple biological mechanisms underlying tumor progression in renal cell carcinoma.
MicroRNA-30a-3p: a potential noncoding RNA target for the treatment of arteriosclerosis obliterans.
Downregulation of HAS‑2 regulates the chondrocyte cytoskeleton and induces cartilage degeneration by activating the RhoA/ROCK signaling pathway.
Nrf2 attenuates methamphetamine-induced myocardial injury by regulating oxidative stress and apoptosis in mice.
Identification of immune-associated signatures and potential therapeutic targets for pulmonary arterial hypertension.
Diosmetin alleviates acute lung injury caused by lipopolysaccharide by targeting barrier function.
The Cytoskeleton Effectors Rho-Kinase (ROCK) and Mammalian Diaphanous-Related (mDia) Formin Have Dynamic Roles in Tumor Microtube Formation in Invasive Glioblastoma Cells.
Oxycodone relieves permeability damage and apoptosis of oxygen-glucose deprivation/reoxygenation-induced brain microvascular endothelial cells through ras homolog family member A (RhoA)/ Rho-associated coiled-coil containing kinases (ROCK)/ myosin light chain 2 (MLC2) signal.
Acquired temozolomide resistance in MGMT(low) gliomas is associated with regulation of homologous recombination repair by ROCK2.
Echinococcus multilocularis drives the polarization of macrophages by regulating the RhoA-MAPK signaling pathway and thus affects liver fibrosis.
Liraglutide Ameliorates Erectile Dysfunction via Regulating Oxidative Stress, the RhoA/ROCK Pathway and Autophagy in Diabetes Mellitus.
JTE-013 supplementation improves erectile dysfunction in rats with streptozotocin-induced type Ⅰ diabetes through the inhibition of the rho-kinase pathway, fibrosis, and apoptosis.
Megakaryocytic Smad4 Regulates Platelet Function through Syk and ROCK2 Expression.
FTY720 Supplementation Partially Improves Erectile Dysfunction in Rats With Streptozotocin-Induced Type 1 Diabetes Through Inhibition of Endothelial Dysfunction and Corporal Fibrosis.
Graphene Oxide Induced Perturbation to Plasma Membrane and Cytoskeletal Meshwork Sensitize Cancer Cells to Chemotherapeutic Agents.
Phosphorylation of nucleophosmin at threonine 234/237 is associated with HCC metastasis.
Rock2 promotes the invasion and metastasis of hepatocellular carcinoma by modifying MMP2 ubiquitination and degradation.
Li J, Liu Y, Chen X, Luo M, Yin M, Xie X, Ai Y, Zhang X, He J
Journal of ethnopharmacology 2024 Jun 28;328:118114
Journal of ethnopharmacology 2024 Jun 28;328:118114
Fasudil attenuates oxidative stress-induced partial epithelial-mesenchymal transition of tubular epithelial cells in hyperuricemic nephropathy via activating Nrf2.
Cao Y, Wang Y, Li W, Feng J, Chen Y, Chen R, Hu L, Wei J
European journal of pharmacology 2024 Jul 15;975:176640
European journal of pharmacology 2024 Jul 15;975:176640
ROCK2-RNA interaction map reveals multiple biological mechanisms underlying tumor progression in renal cell carcinoma.
Hong Z, Chen X, Wang L, Zhou X, He H, Zou G, Liu Q, Wang Y
Human cell 2023 Sep;36(5):1790-1803
Human cell 2023 Sep;36(5):1790-1803
MicroRNA-30a-3p: a potential noncoding RNA target for the treatment of arteriosclerosis obliterans.
Zhang M, Chen Y, Niu F, Luo X, Li J, Hu W
Aging 2023 Oct 27;15(21):11875-11890
Aging 2023 Oct 27;15(21):11875-11890
Downregulation of HAS‑2 regulates the chondrocyte cytoskeleton and induces cartilage degeneration by activating the RhoA/ROCK signaling pathway.
Yang J, Wang L, Zhang Z, Sun Q, Zhang Y
International journal of molecular medicine 2023 Jul;52(1)
International journal of molecular medicine 2023 Jul;52(1)
Nrf2 attenuates methamphetamine-induced myocardial injury by regulating oxidative stress and apoptosis in mice.
Yu H, Peng Y, Dong W, Shen B, Yang G, Nie Q, Tian Y, Qin L, Song C, Chen B, Zhao Y, Li L, Hong S
Human & experimental toxicology 2023 Jan-Dec;42:9603271231219488
Human & experimental toxicology 2023 Jan-Dec;42:9603271231219488
Identification of immune-associated signatures and potential therapeutic targets for pulmonary arterial hypertension.
He X, Fang J, Gong M, Zhang J, Xie R, Zhao D, Gu Y, Ma L, Pang X, Cui Y
Journal of cellular and molecular medicine 2023 Dec;27(23):3864-3877
Journal of cellular and molecular medicine 2023 Dec;27(23):3864-3877
Diosmetin alleviates acute lung injury caused by lipopolysaccharide by targeting barrier function.
Xia J, Li J, Deng M, Yin F, Liu J, Wang J
Inflammopharmacology 2023 Aug;31(4):2037-2047
Inflammopharmacology 2023 Aug;31(4):2037-2047
The Cytoskeleton Effectors Rho-Kinase (ROCK) and Mammalian Diaphanous-Related (mDia) Formin Have Dynamic Roles in Tumor Microtube Formation in Invasive Glioblastoma Cells.
Becker KN, Pettee KM, Sugrue A, Reinard KA, Schroeder JL, Eisenmann KM
Cells 2022 May 5;11(9)
Cells 2022 May 5;11(9)
Oxycodone relieves permeability damage and apoptosis of oxygen-glucose deprivation/reoxygenation-induced brain microvascular endothelial cells through ras homolog family member A (RhoA)/ Rho-associated coiled-coil containing kinases (ROCK)/ myosin light chain 2 (MLC2) signal.
Shao F, Han D, Shen Y, Bian W, Zou L, Hu Y, Sun W
Bioengineered 2022 Mar;13(3):5205-5215
Bioengineered 2022 Mar;13(3):5205-5215
Acquired temozolomide resistance in MGMT(low) gliomas is associated with regulation of homologous recombination repair by ROCK2.
Zhang X, Li T, Yang M, Du Q, Wang R, Fu B, Tan Y, Cao M, Chen Y, Wang Q, Hu R
Cell death & disease 2022 Feb 10;13(2):138
Cell death & disease 2022 Feb 10;13(2):138
Echinococcus multilocularis drives the polarization of macrophages by regulating the RhoA-MAPK signaling pathway and thus affects liver fibrosis.
Chong S, Chen G, Dang Z, Niu F, Zhang L, Ma H, Zhao Y
Bioengineered 2022 Apr;13(4):8747-8758
Bioengineered 2022 Apr;13(4):8747-8758
Liraglutide Ameliorates Erectile Dysfunction via Regulating Oxidative Stress, the RhoA/ROCK Pathway and Autophagy in Diabetes Mellitus.
Yuan P, Ma D, Gao X, Wang J, Li R, Liu Z, Wang T, Wang S, Liu J, Liu X
Frontiers in pharmacology 2020;11:1257
Frontiers in pharmacology 2020;11:1257
JTE-013 supplementation improves erectile dysfunction in rats with streptozotocin-induced type Ⅰ diabetes through the inhibition of the rho-kinase pathway, fibrosis, and apoptosis.
Liu K, Cui K, Feng H, Li R, Lin H, Chen Y, Zhang Y, Chen Z, Yuan H, Li M, Wang T, Lan R, Liu J, Rao K, Wen B
Andrology 2020 Mar;8(2):497-508
Andrology 2020 Mar;8(2):497-508
Megakaryocytic Smad4 Regulates Platelet Function through Syk and ROCK2 Expression.
Wang Y, Jiang L, Mo X, Lan Y, Yang X, Liu X, Zhang J, Zhu L, Liu J, Wu X
Molecular pharmacology 2017 Sep;92(3):285-296
Molecular pharmacology 2017 Sep;92(3):285-296
FTY720 Supplementation Partially Improves Erectile Dysfunction in Rats With Streptozotocin-Induced Type 1 Diabetes Through Inhibition of Endothelial Dysfunction and Corporal Fibrosis.
Cui K, Ruan Y, Wang T, Rao K, Chen Z, Wang S, Liu J
The journal of sexual medicine 2017 Mar;14(3):323-335
The journal of sexual medicine 2017 Mar;14(3):323-335
Graphene Oxide Induced Perturbation to Plasma Membrane and Cytoskeletal Meshwork Sensitize Cancer Cells to Chemotherapeutic Agents.
Zhu J, Xu M, Gao M, Zhang Z, Xu Y, Xia T, Liu S
ACS nano 2017 Mar 28;11(3):2637-2651
ACS nano 2017 Mar 28;11(3):2637-2651
Phosphorylation of nucleophosmin at threonine 234/237 is associated with HCC metastasis.
Ching RH, Lau EY, Ling PM, Lee JM, Ma MK, Cheng BY, Lo RC, Ng IO, Lee TK
Oncotarget 2015 Dec 22;6(41):43483-95
Oncotarget 2015 Dec 22;6(41):43483-95
Rock2 promotes the invasion and metastasis of hepatocellular carcinoma by modifying MMP2 ubiquitination and degradation.
Huang D, Du X, Yuan R, Chen L, Liu T, Wen C, Huang M, Li M, Hao L, Shao J
Biochemical and biophysical research communications 2014 Oct 10;453(1):49-56
Biochemical and biophysical research communications 2014 Oct 10;453(1):49-56
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HT-1080 cells were subjected to SDS PAGE followed by western blot with 21645-1-AP(ROCK2 antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using ROCK2 antibody 21645-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-ROCK2 (IP:21645-1-AP, 5ug; Detection:21645-1-AP 1:700) with HeLa cells lysate 1320ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human liver using 21645-1-AP(ROCK2 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human liver using 21645-1-AP(ROCK2 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
