21645-1-AP

antibody from Proteintech Group

Targeting: ROCK2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

21645-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#21645-1-AP, RRID:AB_10858624

Product name

ROCK2(middle) antibody

Antibody type

Polyclonal

Description

KD/KO validated ROCK2(middle) antibody (Cat. #21645-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Liraglutide Ameliorates Erectile Dysfunction via Regulating Oxidative Stress, the RhoA/ROCK Pathway and Autophagy in Diabetes Mellitus.

Yuan P, Ma D, Gao X, Wang J, Li R, Liu Z, Wang T, Wang S, Liu J, Liu X
Frontiers in pharmacology 2020;11:1257

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JTE-013 supplementation improves erectile dysfunction in rats with streptozotocin-induced type Ⅰ diabetes through the inhibition of the rho-kinase pathway, fibrosis, and apoptosis.

Liu K, Cui K, Feng H, Li R, Lin H, Chen Y, Zhang Y, Chen Z, Yuan H, Li M, Wang T, Lan R, Liu J, Rao K, Wen B
Andrology 2020 Mar;8(2):497-508

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Megakaryocytic Smad4 Regulates Platelet Function through Syk and ROCK2 Expression.

Wang Y, Jiang L, Mo X, Lan Y, Yang X, Liu X, Zhang J, Zhu L, Liu J, Wu X
Molecular pharmacology 2017 Sep;92(3):285-296

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FTY720 Supplementation Partially Improves Erectile Dysfunction in Rats With Streptozotocin-Induced Type 1 Diabetes Through Inhibition of Endothelial Dysfunction and Corporal Fibrosis.

Cui K, Ruan Y, Wang T, Rao K, Chen Z, Wang S, Liu J
The journal of sexual medicine 2017 Mar;14(3):323-335

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Phosphorylation of nucleophosmin at threonine 234/237 is associated with HCC metastasis.

Ching RH, Lau EY, Ling PM, Lee JM, Ma MK, Cheng BY, Lo RC, Ng IO, Lee TK
Oncotarget 2015 Dec 22;6(41):43483-95

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Rock2 promotes the invasion and metastasis of hepatocellular carcinoma by modifying MMP2 ubiquitination and degradation.

Huang D, Du X, Yuan R, Chen L, Liu T, Wen C, Huang M, Li M, Hao L, Shao J
Biochemical and biophysical research communications 2014 Oct 10;453(1):49-56

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HT-1080 cells were subjected to SDS PAGE followed by western blot with 21645-1-AP(ROCK2 antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using ROCK2 antibody 21645-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-ROCK2 (IP:21645-1-AP, 5ug; Detection:21645-1-AP 1:700) with HeLa cells lysate 1320ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver using 21645-1-AP(ROCK2 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human liver using 21645-1-AP(ROCK2 antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue