- GTX122651 - GeneTex ROCK2 antibody

Submitted references Phosphorylation of ARHGAP19 by CDK1 and ROCK regulates its subcellular localization and function during mitosis.

Marceaux C, Petit D, Bertoglio J, David MD
Journal of cell science 2018 Mar 2;131(5)

Atypical Endocannabinoid Signaling Initiates a New Form of Memory-Related Plasticity at a Cortical Input to Hippocampus.

Wang W, Jia Y, Pham DT, Palmer LC, Jung KM, Cox CD, Rumbaugh G, Piomelli D, Gall CM, Lynch G
Cerebral cortex (New York, N.Y. : 1991) 2018 Jul 1;28(7):2253-2266

ARHGEF10 knockout inhibits platelet aggregation and protects mice from thrombus formation.

Lu DH, Hsu CC, Huang SW, Tu HJ, Huang TF, Liou HC, Liao HM, Chen CH, Fu WM, Gau SS
Journal of thrombosis and haemostasis : JTH 2017 Oct;15(10):2053-2064

Induction of endothelium-dependent constriction of mesenteric arteries in endotoxemic hypotensive shock.

Tseng TL, Chen MF, Liu CH, Pang CY, Hsu YH, Lee TJ
British journal of pharmacology 2016 Apr;173(7):1179-95

14-3-3τ promotes breast cancer invasion and metastasis by inhibiting RhoGDIα.

Xiao Y, Lin VY, Ke S, Lin GE, Lin FT, Lin WC
Molecular and cellular biology 2014 Jul;34(14):2635-49

ROCKII Ser1366 phosphorylation reflects the activation status.

Chuang HH, Yang CH, Tsay YG, Hsu CY, Tseng LM, Chang ZF, Lee HH
The Biochemical journal 2012 Apr 1;443(1):145-51

Perturbation of glycoprotein processing affects the neurotoxin-responsive Na+ channel in neuroblastoma cells.

Negishi M, Glick MC
Carbohydrate research 1986 Jun 1;149(1):185-98

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Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 (phospho Ser1366) antibody (GTX122651) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
KD Reagent Type: siRNA
Downregulation: >75%
KD Reagent Prod no: Proprietary.
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod page: KD Reagent Prod page
Antibody Lot Number: 42025

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ROCKII (phospho Ser1366) antibody ?detects the phosphorylation of ROCKII at Ser 1366 residue in IP experiments. HEK293T cells were transfected with wild type (WT) or phospho-mutant (S1366A) ROCKII expression constructs. Overexpressed Myc-ROCKII proteins were immunoprecipitated with anti-myc antibody and probed with anti- ROCKII (phospho Ser1366) antibody (GTX122651, 1:1000 dilution) with or without peptide (0.2 mg/ml) competition as indicated. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Untreated (¡V) and treated (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 (phospho Ser1366) antibody (GTX122651) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (CIP: Calf-intestinal alkaline phosphatase)

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Untreated (¡V) and treated (+) HeLa whole cell extracts (30 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 (phospho Ser1366) antibody (GTX122651) diluted at 1:2000. (CIP: Calf-intestinal alkaline phosphatase)

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 (phospho Ser1366) antibody (GTX122651) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ROCK2 (phospho Ser1366) antibody detects ROCK2 (phospho Ser1366) protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: ROCK2 (phospho Ser1366) protein stained by ROCK2 (phospho Ser1366) antibody (GTX122651) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ROCK2 (phospho Ser1366) antibody detects ROCK2 protein at nucleus and cytosol on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. ROCK2 (phospho Ser1366) antibody (GTX122651) dilution: 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ROCK2 (phospho Ser1366) antibody detects ROCK2 protein at weak nucleus and cytosol on human breast tissue by immunohistochemical analysis. Sample: Paraffin-embedded human breast tissue. ROCK2 (phospho Ser1366) antibody (GTX122651) dilution: 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ROCK2 (phospho Ser1366) antibody detects ROCK2 (phospho Ser1366) protein at cytoplasm and nucleus in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. ROCK2 (phospho Ser1366) antibody (GTX122651) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ROCK2 (phospho Ser1366) antibody detects ROCK2 (phospho Ser1366) protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. ROCK2 (phospho Ser1366) antibody (GTX122651) diluted at 1:500.

GTX122651

Antibody data