Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA503774 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA503774, RRID:AB_11140728
- Product name
- CAPN9 (Calpain 9) mouse monoclonal antibody, clone OTI2G1 (formerly 2G1)
- Antibody type
- Monoclonal
- Description
- CAPN9 (Calpain 9) mouse monoclonal antibody, clone OTI2G1 (formerly 2G1)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CAPN9
- Isotype
- IgG
- Antibody clone number
- OTI2G1
- Vial size
- 100 µl
- Concentration
- 0.78 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CAPN9 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAPN9 (RC215171, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAPN9.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-CAPN9 mouse monoclonal antibody (TA503774) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CAPN9(RC215171).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CAPN9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503774)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CAPN9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503774)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-CAPN9 antibody(TA503774),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
