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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MAB8483 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB8483, RRID:AB_10721496
- Product name
- EGLN1 monoclonal antibody, clone AT1E9
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant EGLN1.
- Isotype
- IgG
- Antibody clone number
- AT1E9
- Storage
- Store at 4°C. For long term storage store at -20°C or lower..Aliquot to avoid repeated freezing and thawing.
Submitted references iTRAQ proteomic identification of pVHL-dependent and -independent targets of Egln1 prolyl hydroxylase knockdown in renal carcinoma cells.
Kinetic characterization and identification of a novel inhibitor of hypoxia-inducible factor prolyl hydroxylase 2 using a time-resolved fluorescence resonance energy transfer-based assay technology.
Defects in embryonic development of EGLN1/PHD2 knockdown transgenic mice are associated with induction of Igfbp in the placenta.
Haffey WD, Mikhaylova O, Meller J, Yi Y, Greis KD, Czyzyk-Krzeska MF
Advances in enzyme regulation 2009;49(1):121-32
Advances in enzyme regulation 2009;49(1):121-32
Kinetic characterization and identification of a novel inhibitor of hypoxia-inducible factor prolyl hydroxylase 2 using a time-resolved fluorescence resonance energy transfer-based assay technology.
Dao JH, Kurzeja RJ, Morachis JM, Veith H, Lewis J, Yu V, Tegley CM, Tagari P
Analytical biochemistry 2009 Jan 15;384(2):213-23
Analytical biochemistry 2009 Jan 15;384(2):213-23
Defects in embryonic development of EGLN1/PHD2 knockdown transgenic mice are associated with induction of Igfbp in the placenta.
Ozolins TR, Fisher TS, Nadeau DM, Stock JL, Klein AS, Milici AJ, Morton D, Wilhelms MB, Brissette WH, Li B
Biochemical and biophysical research communications 2009 Dec 18;390(3):372-6
Biochemical and biophysical research communications 2009 Dec 18;390(3):372-6
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Cell lysates of HeLa (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with EGLN1 monoclonal antibody, clone AT1E9 (Cat # MAB8483) (1 : 1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HeLa cells were stained with EGLN1 monoclonal antibody, clone AT1E9 (Cat # MAB8483) (Green). Nucleus and Actin were stained by Phalloidin-TRITC (Red) or Hoechst 33342 (Blue).
- Validation comment
- Immunofluorescence
