- PA1-16524 - Invitrogen Antibodies EGLN1 antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western Blot analysis of HeLa lysates using Product # PA1-16524.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western Blot analysis of human PHD2, using Product # PA1-16524. Samples: Recombinant FLAG-His-PHD1, PHD2 or PHD3 (10 ng/lane) and whole cell lysate from HeLa cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Knockdown of PHD2 was achieved by transfecting HeLa with PHD2 specific validated siRNAs (Silencer® select Product # s29231). Western blot analysis (Fig. a) was performed using whole cell extracts from the PHD2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with PHD2 Polyclonal Antibody (Product # PA1-16524, 1 µg/mL) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to PHD2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), HEK 293 (Lane 3), COS-7 (Lane 4) and U-87 MG (Lane 5). The blot was probed with Anti-PHD2 Polyclonal Antibody (Product # PA1-16524, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 46 kDa band corresponding to PHD2 was observed across the cell lines tested.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of PHD2 in 10 ng/lane recombinant epitope-tagged PHD1, PHD2 or PHD3 or whole cell lysate from HeLa cells. Samples were incubated in PHD2 polyclonal antibody (Product # PA1-16524 using a dilution of 1 µg/mL. Detection by chemiluminescence.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of PHD2 in 0.5 mg/mL hypoxic HeLa cell lysate. Samples were incubated in PHD2 polyclonal antibody (Product # PA1-16524). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Detection of HIF Prolyl Hydroxylase 2 (Green) in Hela cells using Product # PA1-16524 at a dilution of 1:50. Nuclei (Blue) are counterstainde using Hoechst.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of PHD2 in lung vascular endothelium. Samples were incubated in PHD2 polyclonal antibody (Product # PA1-16524). Image at 40X.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Flow cytometric detection of PHD2. 1 million Jurkat cells were fixed, permeabilized, and stained with 3.0 µg/mL anti-PHD2 (Product # PA1-16524) in a 150 µL reaction. Isotype control (black), anti-MLL1 (red).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of PHD2 in one million Jurkat cells (fixed, permeabilized). Samples were incubated in PHD2 polyclonal (Product # PA1-16524) using a dilution of 3.0 µg/mL (in a 150 µL reaction). Isotype control (black), anti-MLL1 (red).

PA1-16524